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Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents

Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu(2+)...

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Autores principales: Jamdade, Dhiraj A., Rajpali, Dishantsingh, Joshi, Komal A., Kitture, Rohini, Kulkarni, Anuja S., Shinde, Vaishali S., Bellare, Jayesh, Babiya, Kaushik R., Ghosh, Sougata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388358/
https://www.ncbi.nlm.nih.gov/pubmed/30886631
http://dx.doi.org/10.1155/2019/9080279
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author Jamdade, Dhiraj A.
Rajpali, Dishantsingh
Joshi, Komal A.
Kitture, Rohini
Kulkarni, Anuja S.
Shinde, Vaishali S.
Bellare, Jayesh
Babiya, Kaushik R.
Ghosh, Sougata
author_facet Jamdade, Dhiraj A.
Rajpali, Dishantsingh
Joshi, Komal A.
Kitture, Rohini
Kulkarni, Anuja S.
Shinde, Vaishali S.
Bellare, Jayesh
Babiya, Kaushik R.
Ghosh, Sougata
author_sort Jamdade, Dhiraj A.
collection PubMed
description Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu(2+) ions to CuNPs within 5 h at 100°C which were further characterized using UV-visible spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering, X-ray diffraction, and Fourier-transform infrared spectroscopy. Further, the CuNPs were checked for antidiabetic activity using porcine pancreatic α-amylase and α-glucosidase inhibition followed by evaluation of mechanism using circular dichroism spectroscopy. CuNPs were found to be predominantly spherical in nature with a diameter ranging from 1 to 5 nm. The phenolics and flavonoids in the extracts might play a critical role in the synthesis and stabilization process. Significant change in the peak at ∼1095 cm(−1) corresponding to C-O-C bond in ether was observed. CuNPs could inhibit porcine pancreatic α-amylase up to 30% to 50%, while they exhibited a more significant inhibition of α-glucosidase from 70% to 88%. The mechanism of enzyme inhibition was attributed due to the conformational change owing to drastic alteration of secondary structure by CuNPs. This is the first study of its kind that provides a strong scientific rationale that phytogenic CuNPs synthesized using G. glauca and P. zeylanica can be considered to develop candidate antidiabetic nanomedicine.
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spelling pubmed-63883582019-03-18 Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents Jamdade, Dhiraj A. Rajpali, Dishantsingh Joshi, Komal A. Kitture, Rohini Kulkarni, Anuja S. Shinde, Vaishali S. Bellare, Jayesh Babiya, Kaushik R. Ghosh, Sougata Adv Pharmacol Sci Research Article Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu(2+) ions to CuNPs within 5 h at 100°C which were further characterized using UV-visible spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering, X-ray diffraction, and Fourier-transform infrared spectroscopy. Further, the CuNPs were checked for antidiabetic activity using porcine pancreatic α-amylase and α-glucosidase inhibition followed by evaluation of mechanism using circular dichroism spectroscopy. CuNPs were found to be predominantly spherical in nature with a diameter ranging from 1 to 5 nm. The phenolics and flavonoids in the extracts might play a critical role in the synthesis and stabilization process. Significant change in the peak at ∼1095 cm(−1) corresponding to C-O-C bond in ether was observed. CuNPs could inhibit porcine pancreatic α-amylase up to 30% to 50%, while they exhibited a more significant inhibition of α-glucosidase from 70% to 88%. The mechanism of enzyme inhibition was attributed due to the conformational change owing to drastic alteration of secondary structure by CuNPs. This is the first study of its kind that provides a strong scientific rationale that phytogenic CuNPs synthesized using G. glauca and P. zeylanica can be considered to develop candidate antidiabetic nanomedicine. Hindawi 2019-02-11 /pmc/articles/PMC6388358/ /pubmed/30886631 http://dx.doi.org/10.1155/2019/9080279 Text en Copyright © 2019 Dhiraj A. Jamdade et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jamdade, Dhiraj A.
Rajpali, Dishantsingh
Joshi, Komal A.
Kitture, Rohini
Kulkarni, Anuja S.
Shinde, Vaishali S.
Bellare, Jayesh
Babiya, Kaushik R.
Ghosh, Sougata
Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title_full Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title_fullStr Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title_full_unstemmed Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title_short Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
title_sort gnidia glauca- and plumbago zeylanica-mediated synthesis of novel copper nanoparticles as promising antidiabetic agents
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388358/
https://www.ncbi.nlm.nih.gov/pubmed/30886631
http://dx.doi.org/10.1155/2019/9080279
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