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Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage

BACKGROUND: Dicerandrol B is a natural antitumor agent that can be isolated from the endophytic fungus, Phomopsis sp. The present study investigated the effects of dicerandrol B on human cervical cancer HeLa cells. MATERIALS AND METHODS: In this study, dicerandrol B was identified by electrospray io...

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Autores principales: Gao, Dandan, Guo, Zhimin, Wang, Jiabin, Hu, Gaofeng, Su, Yuqiao, Chen, Lijun, Lv, Qianwen, Yu, Huimei, Qin, Jianchun, Xu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388992/
https://www.ncbi.nlm.nih.gov/pubmed/30863086
http://dx.doi.org/10.2147/OTT.S191204
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author Gao, Dandan
Guo, Zhimin
Wang, Jiabin
Hu, Gaofeng
Su, Yuqiao
Chen, Lijun
Lv, Qianwen
Yu, Huimei
Qin, Jianchun
Xu, Wei
author_facet Gao, Dandan
Guo, Zhimin
Wang, Jiabin
Hu, Gaofeng
Su, Yuqiao
Chen, Lijun
Lv, Qianwen
Yu, Huimei
Qin, Jianchun
Xu, Wei
author_sort Gao, Dandan
collection PubMed
description BACKGROUND: Dicerandrol B is a natural antitumor agent that can be isolated from the endophytic fungus, Phomopsis sp. The present study investigated the effects of dicerandrol B on human cervical cancer HeLa cells. MATERIALS AND METHODS: In this study, dicerandrol B was identified by electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. We used MTT to detect the cell viability. Flow cytometry was used to analyze the apoptosis and cell cycle. Western blot was used to examine the expression of related proteins. RESULTS: Dicerandrol B was isolated from the endophytic fungus Phomopsis sp. The MTT assay and flow cytometry showed that dicerandrol B significantly inhibited HeLa cell viability and induced G2/M cell cycle arrest. Western blot analysis demonstrated that dicerandrol B increased the levels of GRP78, ubiquitin, cleaved PARP, and Bax protein, decreased the levels of PARP and Bcl-2 protein, and caused an increase in the Bax/Bcl-2 ratio in HeLa cells. Dicerandrol B increased the production of ROS in HeLa cells, which was attenuated by the antioxidant N-acetyl-l-cysteine. CONCLUSION: These findings suggest that dicerandrol B induces apoptosis in human HeLa cells, possibly through the endoplasmic reticulum stress and mitochondrial apoptotic pathways. This suggests that dicerandrol B possesses strong anticancer activity in cervical cancer and provides insight into the underlying mechanisms.
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spelling pubmed-63889922019-03-12 Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage Gao, Dandan Guo, Zhimin Wang, Jiabin Hu, Gaofeng Su, Yuqiao Chen, Lijun Lv, Qianwen Yu, Huimei Qin, Jianchun Xu, Wei Onco Targets Ther Original Research BACKGROUND: Dicerandrol B is a natural antitumor agent that can be isolated from the endophytic fungus, Phomopsis sp. The present study investigated the effects of dicerandrol B on human cervical cancer HeLa cells. MATERIALS AND METHODS: In this study, dicerandrol B was identified by electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. We used MTT to detect the cell viability. Flow cytometry was used to analyze the apoptosis and cell cycle. Western blot was used to examine the expression of related proteins. RESULTS: Dicerandrol B was isolated from the endophytic fungus Phomopsis sp. The MTT assay and flow cytometry showed that dicerandrol B significantly inhibited HeLa cell viability and induced G2/M cell cycle arrest. Western blot analysis demonstrated that dicerandrol B increased the levels of GRP78, ubiquitin, cleaved PARP, and Bax protein, decreased the levels of PARP and Bcl-2 protein, and caused an increase in the Bax/Bcl-2 ratio in HeLa cells. Dicerandrol B increased the production of ROS in HeLa cells, which was attenuated by the antioxidant N-acetyl-l-cysteine. CONCLUSION: These findings suggest that dicerandrol B induces apoptosis in human HeLa cells, possibly through the endoplasmic reticulum stress and mitochondrial apoptotic pathways. This suggests that dicerandrol B possesses strong anticancer activity in cervical cancer and provides insight into the underlying mechanisms. Dove Medical Press 2019-02-13 /pmc/articles/PMC6388992/ /pubmed/30863086 http://dx.doi.org/10.2147/OTT.S191204 Text en © 2019 Gao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Gao, Dandan
Guo, Zhimin
Wang, Jiabin
Hu, Gaofeng
Su, Yuqiao
Chen, Lijun
Lv, Qianwen
Yu, Huimei
Qin, Jianchun
Xu, Wei
Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title_full Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title_fullStr Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title_full_unstemmed Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title_short Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
title_sort dicerandrol b: a natural xanthone dimer induces apoptosis in cervical cancer hela cells through the endoplasmic reticulum stress and mitochondrial damage
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388992/
https://www.ncbi.nlm.nih.gov/pubmed/30863086
http://dx.doi.org/10.2147/OTT.S191204
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