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Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage

BACKGROUND: The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9) has been considerably decreased by rationally engineered variants with increased fidelity (eSpCas9; SpCas9-HF1). However, a subset of targets still generate considerable off-target effects. To deal specificall...

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Detalles Bibliográficos
Autores principales:	Kulcsár, Péter István, Tálas, András, Huszár, Krisztina, Ligeti, Zoltán, Tóth, Eszter, Weinhardt, Nóra, Fodor, Elfrieda, Welker, Ervin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389135/ https://www.ncbi.nlm.nih.gov/pubmed/28985763 http://dx.doi.org/10.1186/s13059-017-1318-8

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