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Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice

Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicine. The ai...

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Autores principales: Luo, Yanping, Zhang, Guochao, Liu, Xun, Yuan, Miaomiao, Gao, Qi, Gao, Haijun, Ke, Lixin, Zhang, Xinxing, Shi, Yanbin, Ma, Xingming, Zhang, Lifeng, Dong, Kaizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389144/
https://www.ncbi.nlm.nih.gov/pubmed/30286809
http://dx.doi.org/10.1186/s13567-018-0596-9
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author Luo, Yanping
Zhang, Guochao
Liu, Xun
Yuan, Miaomiao
Gao, Qi
Gao, Haijun
Ke, Lixin
Zhang, Xinxing
Shi, Yanbin
Ma, Xingming
Zhang, Lifeng
Dong, Kaizhong
author_facet Luo, Yanping
Zhang, Guochao
Liu, Xun
Yuan, Miaomiao
Gao, Qi
Gao, Haijun
Ke, Lixin
Zhang, Xinxing
Shi, Yanbin
Ma, Xingming
Zhang, Lifeng
Dong, Kaizhong
author_sort Luo, Yanping
collection PubMed
description Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicine. The aim of this study was to screen insecticidal water-soluble alkaloids from S. moorcroftiana seeds and evaluate the therapeutic effects against CE and the immune response induced by the alkaloidal fraction. Low polarity compounds (E2-a) were isolated from water-soluble alkaloid (E2) and matrine and sophocarpine were identified as major components. The E2-a fraction was more effective against protoscoleces than other constituents from S. moorcroftiana. After 20 weeks of secondary infection with protoscoleces, mice were orally treated with E2-a (100 mg/kg/day) for 6 weeks to evaluate therapeutic and immunoregulatory activities. Compared with the untreated group, E2-a treatment induced a significant reduction in cyst weight (mean 2.93 g) (p < 0.05) and an impaired ultrastructural modification of the cyst. Interestingly, the application of E2-a resulted in a significant increased frequency of CD3(+)CD4(+) T-cell subsets and decreased frequency of CD3(+)PD-1(+) T-cell subsets, compared with protoscolece-infected mice without treatment. The E2-a fraction of S. moorcroftiana can inhibit the cyst development of CE and boost the specific immune response by reducing the expression of PD-1 and accelerate the cytokine secretion of antigen-specific T-cells. All data suggest the E2-a fraction from S. moorcroftiana seeds may be used as a new potential therapeutic option against E. granulosus infection.
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spelling pubmed-63891442019-03-19 Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice Luo, Yanping Zhang, Guochao Liu, Xun Yuan, Miaomiao Gao, Qi Gao, Haijun Ke, Lixin Zhang, Xinxing Shi, Yanbin Ma, Xingming Zhang, Lifeng Dong, Kaizhong Vet Res Research Article Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicine. The aim of this study was to screen insecticidal water-soluble alkaloids from S. moorcroftiana seeds and evaluate the therapeutic effects against CE and the immune response induced by the alkaloidal fraction. Low polarity compounds (E2-a) were isolated from water-soluble alkaloid (E2) and matrine and sophocarpine were identified as major components. The E2-a fraction was more effective against protoscoleces than other constituents from S. moorcroftiana. After 20 weeks of secondary infection with protoscoleces, mice were orally treated with E2-a (100 mg/kg/day) for 6 weeks to evaluate therapeutic and immunoregulatory activities. Compared with the untreated group, E2-a treatment induced a significant reduction in cyst weight (mean 2.93 g) (p < 0.05) and an impaired ultrastructural modification of the cyst. Interestingly, the application of E2-a resulted in a significant increased frequency of CD3(+)CD4(+) T-cell subsets and decreased frequency of CD3(+)PD-1(+) T-cell subsets, compared with protoscolece-infected mice without treatment. The E2-a fraction of S. moorcroftiana can inhibit the cyst development of CE and boost the specific immune response by reducing the expression of PD-1 and accelerate the cytokine secretion of antigen-specific T-cells. All data suggest the E2-a fraction from S. moorcroftiana seeds may be used as a new potential therapeutic option against E. granulosus infection. BioMed Central 2018-10-04 2018 /pmc/articles/PMC6389144/ /pubmed/30286809 http://dx.doi.org/10.1186/s13567-018-0596-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Luo, Yanping
Zhang, Guochao
Liu, Xun
Yuan, Miaomiao
Gao, Qi
Gao, Haijun
Ke, Lixin
Zhang, Xinxing
Shi, Yanbin
Ma, Xingming
Zhang, Lifeng
Dong, Kaizhong
Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title_full Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title_fullStr Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title_full_unstemmed Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title_short Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
title_sort therapeutic and immunoregulatory effects of water-soluble alkaloids e2-a from sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389144/
https://www.ncbi.nlm.nih.gov/pubmed/30286809
http://dx.doi.org/10.1186/s13567-018-0596-9
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