Cargando…

Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles

BACKGROUND: Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Pengfei, Meng, Jinzhu, Jing, Jiongjie, Hao, Qingling, Zhu, Zhiwei, Yao, Jianbo, Lyu, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389156/
https://www.ncbi.nlm.nih.gov/pubmed/29482665
http://dx.doi.org/10.1186/s40659-018-0154-y
_version_ 1783397900833783808
author Li, Pengfei
Meng, Jinzhu
Jing, Jiongjie
Hao, Qingling
Zhu, Zhiwei
Yao, Jianbo
Lyu, Lihua
author_facet Li, Pengfei
Meng, Jinzhu
Jing, Jiongjie
Hao, Qingling
Zhu, Zhiwei
Yao, Jianbo
Lyu, Lihua
author_sort Li, Pengfei
collection PubMed
description BACKGROUND: Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Further research found that secretion of E(2) was inhibited in granulosa cells with a certain dose of CART, the effect depends on the stage of cell differentiation, suggesting that CART could play a crucial role in regulating follicle atresia. The objective of this study was to characterize the CART expression model and hormones secretion in vivo and vitro in pig follicle granulosa cells, preliminarily studied whether CART have an effect on granulosa cells proliferation and hormones secretion in multiparous animals such as pigs. METHODS: The expression levels of CART mRNA in granulosa cells of different follicles were analyzed using qRT-PCR technology. Immunohistochemistry technology was used to localize CART peptide. Granulosa cells were cultured in medium supplemented with different concentrations of CART and FSH for 168 h using Long-term culture system, and observed using a microscope. The concentration of Estradiol (E(2)) and progesterone (P) in follicular fluids of different test groups were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Results showed that expression level of CART mRNA was highest in medium follicles, and significantly higher than that in large and small follicles (P < 0.05). Immunohistochemical results showed that CART were expressed both in granulosa cells and theca cells of large follicles, while CART were detected only in theca cells of medium and small follicles. After the granulosa cells were cultured for 168 h, and found that concentrations of E(2) increase with concentrations of follicle-stimulating hormone (FSH) increase when the CART concentration was 0 μM. And the concentration of FSH reached 25 ng/mL, the concentration of E(2) is greatest. It shows that the production of E(2) needs induction of FSH in granulosa cells of pig ovarian follicles. With the increasing of CART concentrations (0.01, 0.1, 1 μM), E(2) concentration has a declining trend, when the FSH concentrations were 25 and 50 ng/mL in the medium, respectively. CONCLUSIONS: These results suggested that CART plays a role to inhibit granulosa cells proliferation and E(2) production, which induced by FSH in porcine ovarian follicular granulosa cells in vitro, but the inhibition effect is not significant. So we hypothesis CART maybe not a main local negative regulatory factor during porcine follicular development, which is different from the single fetal animals.
format Online
Article
Text
id pubmed-6389156
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-63891562019-03-19 Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles Li, Pengfei Meng, Jinzhu Jing, Jiongjie Hao, Qingling Zhu, Zhiwei Yao, Jianbo Lyu, Lihua Biol Res Research Article BACKGROUND: Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Further research found that secretion of E(2) was inhibited in granulosa cells with a certain dose of CART, the effect depends on the stage of cell differentiation, suggesting that CART could play a crucial role in regulating follicle atresia. The objective of this study was to characterize the CART expression model and hormones secretion in vivo and vitro in pig follicle granulosa cells, preliminarily studied whether CART have an effect on granulosa cells proliferation and hormones secretion in multiparous animals such as pigs. METHODS: The expression levels of CART mRNA in granulosa cells of different follicles were analyzed using qRT-PCR technology. Immunohistochemistry technology was used to localize CART peptide. Granulosa cells were cultured in medium supplemented with different concentrations of CART and FSH for 168 h using Long-term culture system, and observed using a microscope. The concentration of Estradiol (E(2)) and progesterone (P) in follicular fluids of different test groups were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Results showed that expression level of CART mRNA was highest in medium follicles, and significantly higher than that in large and small follicles (P < 0.05). Immunohistochemical results showed that CART were expressed both in granulosa cells and theca cells of large follicles, while CART were detected only in theca cells of medium and small follicles. After the granulosa cells were cultured for 168 h, and found that concentrations of E(2) increase with concentrations of follicle-stimulating hormone (FSH) increase when the CART concentration was 0 μM. And the concentration of FSH reached 25 ng/mL, the concentration of E(2) is greatest. It shows that the production of E(2) needs induction of FSH in granulosa cells of pig ovarian follicles. With the increasing of CART concentrations (0.01, 0.1, 1 μM), E(2) concentration has a declining trend, when the FSH concentrations were 25 and 50 ng/mL in the medium, respectively. CONCLUSIONS: These results suggested that CART plays a role to inhibit granulosa cells proliferation and E(2) production, which induced by FSH in porcine ovarian follicular granulosa cells in vitro, but the inhibition effect is not significant. So we hypothesis CART maybe not a main local negative regulatory factor during porcine follicular development, which is different from the single fetal animals. BioMed Central 2018-02-26 /pmc/articles/PMC6389156/ /pubmed/29482665 http://dx.doi.org/10.1186/s40659-018-0154-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Pengfei
Meng, Jinzhu
Jing, Jiongjie
Hao, Qingling
Zhu, Zhiwei
Yao, Jianbo
Lyu, Lihua
Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title_full Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title_fullStr Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title_full_unstemmed Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title_short Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
title_sort study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389156/
https://www.ncbi.nlm.nih.gov/pubmed/29482665
http://dx.doi.org/10.1186/s40659-018-0154-y
work_keys_str_mv AT lipengfei studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT mengjinzhu studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT jingjiongjie studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT haoqingling studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT zhuzhiwei studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT yaojianbo studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles
AT lyulihua studyontherelationshipbetweenexpressionpatternsofcocaineandamphetamineregulatedtranscriptandhormonessecretioninporcineovarianfollicles