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Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens

The respiratory tract is a key organ for many avian pathogens as well as a major route for vaccination in the poultry industry. To improve immune responses after vaccination of chickens through increased uptake of vaccines and targeting to antigen presenting cells, a better understanding of the avia...

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Autores principales: Sutton, Kate, Costa, Taiana, Alber, Andreas, Bryson, Karen, Borowska, Dominika, Balic, Adam, Kaiser, Pete, Stevens, Mark, Vervelde, Lonneke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389226/
https://www.ncbi.nlm.nih.gov/pubmed/30305141
http://dx.doi.org/10.1186/s13567-018-0598-7
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author Sutton, Kate
Costa, Taiana
Alber, Andreas
Bryson, Karen
Borowska, Dominika
Balic, Adam
Kaiser, Pete
Stevens, Mark
Vervelde, Lonneke
author_facet Sutton, Kate
Costa, Taiana
Alber, Andreas
Bryson, Karen
Borowska, Dominika
Balic, Adam
Kaiser, Pete
Stevens, Mark
Vervelde, Lonneke
author_sort Sutton, Kate
collection PubMed
description The respiratory tract is a key organ for many avian pathogens as well as a major route for vaccination in the poultry industry. To improve immune responses after vaccination of chickens through increased uptake of vaccines and targeting to antigen presenting cells, a better understanding of the avian respiratory immune system is required. Transgenic MacReporter birds were used expressing a reporter gene (eGFP or mApple) under the control of the CSF1R promoter and enhancer in cells of the mononuclear phagocyte (MNP) lineage to visualize the ontogeny of the lymphoid tissue, macrophages and dendritic cells, in the trachea, lung and air sac of birds from embryonic day 18–63 weeks of age. Small aggregates of CSF1R-transgene(+) cells start to form at the openings of the secondary bronchi at 1 week of age, indicative of the early development of the organised bronchus-associated lymphoid tissue. Immunohistochemical staining revealed subpopulations of MNPs in the lung, based on expression of CSF1R-transgene, CD11, TIM4, LAMP1, and MHC II. Specialised epithelial cells or M cells covering the bronchus-associated lymphoid tissue expressed CSF1R-transgene and type II pneumocytes expressed LAMP1 suggesting that these epithelial cells are phagocytic and transcytose antigen. Highly organised lymphoid tissue was seen in trachea from 4 weeks onwards. Throughout the air sacs at all ages, CSF1R-transgene(+) cells were scattered and at later stages, CSF1R-transgene(+) cells lined capillaries. These results will serve as a base for further functional characterization of macrophages and dendritic cells and their role in respiratory diseases and vaccine responses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0598-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-63892262019-03-19 Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens Sutton, Kate Costa, Taiana Alber, Andreas Bryson, Karen Borowska, Dominika Balic, Adam Kaiser, Pete Stevens, Mark Vervelde, Lonneke Vet Res Research Article The respiratory tract is a key organ for many avian pathogens as well as a major route for vaccination in the poultry industry. To improve immune responses after vaccination of chickens through increased uptake of vaccines and targeting to antigen presenting cells, a better understanding of the avian respiratory immune system is required. Transgenic MacReporter birds were used expressing a reporter gene (eGFP or mApple) under the control of the CSF1R promoter and enhancer in cells of the mononuclear phagocyte (MNP) lineage to visualize the ontogeny of the lymphoid tissue, macrophages and dendritic cells, in the trachea, lung and air sac of birds from embryonic day 18–63 weeks of age. Small aggregates of CSF1R-transgene(+) cells start to form at the openings of the secondary bronchi at 1 week of age, indicative of the early development of the organised bronchus-associated lymphoid tissue. Immunohistochemical staining revealed subpopulations of MNPs in the lung, based on expression of CSF1R-transgene, CD11, TIM4, LAMP1, and MHC II. Specialised epithelial cells or M cells covering the bronchus-associated lymphoid tissue expressed CSF1R-transgene and type II pneumocytes expressed LAMP1 suggesting that these epithelial cells are phagocytic and transcytose antigen. Highly organised lymphoid tissue was seen in trachea from 4 weeks onwards. Throughout the air sacs at all ages, CSF1R-transgene(+) cells were scattered and at later stages, CSF1R-transgene(+) cells lined capillaries. These results will serve as a base for further functional characterization of macrophages and dendritic cells and their role in respiratory diseases and vaccine responses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0598-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-10 2018 /pmc/articles/PMC6389226/ /pubmed/30305141 http://dx.doi.org/10.1186/s13567-018-0598-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sutton, Kate
Costa, Taiana
Alber, Andreas
Bryson, Karen
Borowska, Dominika
Balic, Adam
Kaiser, Pete
Stevens, Mark
Vervelde, Lonneke
Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title_full Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title_fullStr Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title_full_unstemmed Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title_short Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens
title_sort visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using csf1r-transgenic chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389226/
https://www.ncbi.nlm.nih.gov/pubmed/30305141
http://dx.doi.org/10.1186/s13567-018-0598-7
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