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Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms
Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389966/ https://www.ncbi.nlm.nih.gov/pubmed/30804362 http://dx.doi.org/10.1038/s41598-019-38968-y |
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author | Kiamco, Mia Mae Zmuda, Hannah M. Mohamed, Abdelrhman Call, Douglas R. Raval, Yash S. Patel, Robin Beyenal, Haluk |
author_facet | Kiamco, Mia Mae Zmuda, Hannah M. Mohamed, Abdelrhman Call, Douglas R. Raval, Yash S. Patel, Robin Beyenal, Haluk |
author_sort | Kiamco, Mia Mae |
collection | PubMed |
description | Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue. Production of HOCl near the e-scaffold surface was verified by measuring its concentration using needle-type microelectrodes. E-scaffolds producing 17, 10 and 7 mM HOCl completely eradicated S. aureus, A. baumannii, and P. aeruginosa biofilms after 3 hours, 2 hours, and 1 hour, respectively. Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. The described strategy may provide a novel antibiotic-free strategy for treating persistent biofilm-associated infections, such as wound infections. |
format | Online Article Text |
id | pubmed-6389966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63899662019-02-28 Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms Kiamco, Mia Mae Zmuda, Hannah M. Mohamed, Abdelrhman Call, Douglas R. Raval, Yash S. Patel, Robin Beyenal, Haluk Sci Rep Article Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue. Production of HOCl near the e-scaffold surface was verified by measuring its concentration using needle-type microelectrodes. E-scaffolds producing 17, 10 and 7 mM HOCl completely eradicated S. aureus, A. baumannii, and P. aeruginosa biofilms after 3 hours, 2 hours, and 1 hour, respectively. Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. The described strategy may provide a novel antibiotic-free strategy for treating persistent biofilm-associated infections, such as wound infections. Nature Publishing Group UK 2019-02-25 /pmc/articles/PMC6389966/ /pubmed/30804362 http://dx.doi.org/10.1038/s41598-019-38968-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kiamco, Mia Mae Zmuda, Hannah M. Mohamed, Abdelrhman Call, Douglas R. Raval, Yash S. Patel, Robin Beyenal, Haluk Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title | Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title_full | Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title_fullStr | Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title_full_unstemmed | Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title_short | Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms |
title_sort | hypochlorous-acid-generating electrochemical scaffold for treatment of wound biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389966/ https://www.ncbi.nlm.nih.gov/pubmed/30804362 http://dx.doi.org/10.1038/s41598-019-38968-y |
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