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Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity

Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice...

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Autores principales: Jung, Hyo Young, Yoo, Dae Young, Nam, Sung Min, Kim, Jong Whi, Kim, Woosuk, Kwon, Hyun Jung, Lee, Kwon Young, Choi, Jung Hoon, Kim, Dae Won, Yoon, Yeo Sung, Seong, Je Kyung, Hwang, In Koo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390017/
https://www.ncbi.nlm.nih.gov/pubmed/30664214
http://dx.doi.org/10.3892/mmr.2019.9867
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author Jung, Hyo Young
Yoo, Dae Young
Nam, Sung Min
Kim, Jong Whi
Kim, Woosuk
Kwon, Hyun Jung
Lee, Kwon Young
Choi, Jung Hoon
Kim, Dae Won
Yoon, Yeo Sung
Seong, Je Kyung
Hwang, In Koo
author_facet Jung, Hyo Young
Yoo, Dae Young
Nam, Sung Min
Kim, Jong Whi
Kim, Woosuk
Kwon, Hyun Jung
Lee, Kwon Young
Choi, Jung Hoon
Kim, Dae Won
Yoon, Yeo Sung
Seong, Je Kyung
Hwang, In Koo
author_sort Jung, Hyo Young
collection PubMed
description Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice at postnatal days (P) 1, 7, 14, 28, and 56. In addition, the presented study examined the effects of COX-2 on synaptic plasticity through Arc, phosphorylated cAMP response element-binding protein (pCREB), N-methyl-d-aspartate receptor 1 (GluN1), and GluN2A/2B immunohistochemistry, which was performed on COX-2 knockout (KO) and wild-type (WT) mice. Extremely weak COX-2 immunoreactivity was detected in the hippocampal CA1-3 areas in addition to the dentate gyrus at P1. Conversely, COX-2 immunoreactivity was observed in the stratum pyramidale of the CA1-3 regions and in the outer granule cell layer of the dentate gyrus at P7. Additionally, although peak COX-2 immunoreactivity was observed in all hippocampal sub-regions, including the dentate gyrus at P14, it was significantly decreased at P14. Finally, COX-2 immunoreactivity and the distribution pattern seen at P56 in the hippocampal CA1-3 regions were similar to those observed at P28, whereas, they were identified in the inner half of the granule cell layer of the dentate gyrus. The western blot analysis revealed that the COX-2 protein levels peaked at P14 and were decreased at P28 and P56. Additionally, the number of Arc and pCREB immunoreactive cells as well as GluN1 and GluN2A/2B immunoreactivity of COX-2 KO mice were significantly decreased in the dentate gyrus when compared with that in WT mice. Taken together, the results of the present study suggest that COX-2 serves an important role in synaptic plasticity in the dentate gyrus and changes in the levels of its constitutive expression are associated with the hippocampal dentate gyrus postnatal development.
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spelling pubmed-63900172019-03-07 Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity Jung, Hyo Young Yoo, Dae Young Nam, Sung Min Kim, Jong Whi Kim, Woosuk Kwon, Hyun Jung Lee, Kwon Young Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Seong, Je Kyung Hwang, In Koo Mol Med Rep Articles Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice at postnatal days (P) 1, 7, 14, 28, and 56. In addition, the presented study examined the effects of COX-2 on synaptic plasticity through Arc, phosphorylated cAMP response element-binding protein (pCREB), N-methyl-d-aspartate receptor 1 (GluN1), and GluN2A/2B immunohistochemistry, which was performed on COX-2 knockout (KO) and wild-type (WT) mice. Extremely weak COX-2 immunoreactivity was detected in the hippocampal CA1-3 areas in addition to the dentate gyrus at P1. Conversely, COX-2 immunoreactivity was observed in the stratum pyramidale of the CA1-3 regions and in the outer granule cell layer of the dentate gyrus at P7. Additionally, although peak COX-2 immunoreactivity was observed in all hippocampal sub-regions, including the dentate gyrus at P14, it was significantly decreased at P14. Finally, COX-2 immunoreactivity and the distribution pattern seen at P56 in the hippocampal CA1-3 regions were similar to those observed at P28, whereas, they were identified in the inner half of the granule cell layer of the dentate gyrus. The western blot analysis revealed that the COX-2 protein levels peaked at P14 and were decreased at P28 and P56. Additionally, the number of Arc and pCREB immunoreactive cells as well as GluN1 and GluN2A/2B immunoreactivity of COX-2 KO mice were significantly decreased in the dentate gyrus when compared with that in WT mice. Taken together, the results of the present study suggest that COX-2 serves an important role in synaptic plasticity in the dentate gyrus and changes in the levels of its constitutive expression are associated with the hippocampal dentate gyrus postnatal development. D.A. Spandidos 2019-03 2019-01-15 /pmc/articles/PMC6390017/ /pubmed/30664214 http://dx.doi.org/10.3892/mmr.2019.9867 Text en Copyright: © Jung et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Jung, Hyo Young
Yoo, Dae Young
Nam, Sung Min
Kim, Jong Whi
Kim, Woosuk
Kwon, Hyun Jung
Lee, Kwon Young
Choi, Jung Hoon
Kim, Dae Won
Yoon, Yeo Sung
Seong, Je Kyung
Hwang, In Koo
Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title_full Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title_fullStr Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title_full_unstemmed Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title_short Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
title_sort postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390017/
https://www.ncbi.nlm.nih.gov/pubmed/30664214
http://dx.doi.org/10.3892/mmr.2019.9867
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