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Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity
Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390017/ https://www.ncbi.nlm.nih.gov/pubmed/30664214 http://dx.doi.org/10.3892/mmr.2019.9867 |
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author | Jung, Hyo Young Yoo, Dae Young Nam, Sung Min Kim, Jong Whi Kim, Woosuk Kwon, Hyun Jung Lee, Kwon Young Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Seong, Je Kyung Hwang, In Koo |
author_facet | Jung, Hyo Young Yoo, Dae Young Nam, Sung Min Kim, Jong Whi Kim, Woosuk Kwon, Hyun Jung Lee, Kwon Young Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Seong, Je Kyung Hwang, In Koo |
author_sort | Jung, Hyo Young |
collection | PubMed |
description | Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice at postnatal days (P) 1, 7, 14, 28, and 56. In addition, the presented study examined the effects of COX-2 on synaptic plasticity through Arc, phosphorylated cAMP response element-binding protein (pCREB), N-methyl-d-aspartate receptor 1 (GluN1), and GluN2A/2B immunohistochemistry, which was performed on COX-2 knockout (KO) and wild-type (WT) mice. Extremely weak COX-2 immunoreactivity was detected in the hippocampal CA1-3 areas in addition to the dentate gyrus at P1. Conversely, COX-2 immunoreactivity was observed in the stratum pyramidale of the CA1-3 regions and in the outer granule cell layer of the dentate gyrus at P7. Additionally, although peak COX-2 immunoreactivity was observed in all hippocampal sub-regions, including the dentate gyrus at P14, it was significantly decreased at P14. Finally, COX-2 immunoreactivity and the distribution pattern seen at P56 in the hippocampal CA1-3 regions were similar to those observed at P28, whereas, they were identified in the inner half of the granule cell layer of the dentate gyrus. The western blot analysis revealed that the COX-2 protein levels peaked at P14 and were decreased at P28 and P56. Additionally, the number of Arc and pCREB immunoreactive cells as well as GluN1 and GluN2A/2B immunoreactivity of COX-2 KO mice were significantly decreased in the dentate gyrus when compared with that in WT mice. Taken together, the results of the present study suggest that COX-2 serves an important role in synaptic plasticity in the dentate gyrus and changes in the levels of its constitutive expression are associated with the hippocampal dentate gyrus postnatal development. |
format | Online Article Text |
id | pubmed-6390017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63900172019-03-07 Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity Jung, Hyo Young Yoo, Dae Young Nam, Sung Min Kim, Jong Whi Kim, Woosuk Kwon, Hyun Jung Lee, Kwon Young Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Seong, Je Kyung Hwang, In Koo Mol Med Rep Articles Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice at postnatal days (P) 1, 7, 14, 28, and 56. In addition, the presented study examined the effects of COX-2 on synaptic plasticity through Arc, phosphorylated cAMP response element-binding protein (pCREB), N-methyl-d-aspartate receptor 1 (GluN1), and GluN2A/2B immunohistochemistry, which was performed on COX-2 knockout (KO) and wild-type (WT) mice. Extremely weak COX-2 immunoreactivity was detected in the hippocampal CA1-3 areas in addition to the dentate gyrus at P1. Conversely, COX-2 immunoreactivity was observed in the stratum pyramidale of the CA1-3 regions and in the outer granule cell layer of the dentate gyrus at P7. Additionally, although peak COX-2 immunoreactivity was observed in all hippocampal sub-regions, including the dentate gyrus at P14, it was significantly decreased at P14. Finally, COX-2 immunoreactivity and the distribution pattern seen at P56 in the hippocampal CA1-3 regions were similar to those observed at P28, whereas, they were identified in the inner half of the granule cell layer of the dentate gyrus. The western blot analysis revealed that the COX-2 protein levels peaked at P14 and were decreased at P28 and P56. Additionally, the number of Arc and pCREB immunoreactive cells as well as GluN1 and GluN2A/2B immunoreactivity of COX-2 KO mice were significantly decreased in the dentate gyrus when compared with that in WT mice. Taken together, the results of the present study suggest that COX-2 serves an important role in synaptic plasticity in the dentate gyrus and changes in the levels of its constitutive expression are associated with the hippocampal dentate gyrus postnatal development. D.A. Spandidos 2019-03 2019-01-15 /pmc/articles/PMC6390017/ /pubmed/30664214 http://dx.doi.org/10.3892/mmr.2019.9867 Text en Copyright: © Jung et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Jung, Hyo Young Yoo, Dae Young Nam, Sung Min Kim, Jong Whi Kim, Woosuk Kwon, Hyun Jung Lee, Kwon Young Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Seong, Je Kyung Hwang, In Koo Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title | Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title_full | Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title_fullStr | Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title_full_unstemmed | Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title_short | Postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
title_sort | postnatal changes in constitutive cyclooxygenase-2 expression in the mice hippocampus and its function in synaptic plasticity |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390017/ https://www.ncbi.nlm.nih.gov/pubmed/30664214 http://dx.doi.org/10.3892/mmr.2019.9867 |
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