Cargando…

ATP synthase is required for male fertility and germ cell maturation in Drosophila testes

Germ cell maturation is essential for spermatogenesis and testis homeostasis. ATP synthase serves significant roles in energy storage in germ cell survival and is catalyzed by alterations in the mitochondrial membrane proton concentration. The intrinsic cellular mechanisms governing stem cell matura...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Jun, Chen, Binghai, Zheng, Bo, Qiao, Chen, Chen, Xia, Yan, Yidan, Luan, Xiaojin, Xie, Bing, Liu, Jiajia, Shen, Cong, He, Zeyu, Hu, Xing, Liu, Mingxi, Li, Hong, Shao, Qixiang, Fang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390039/
https://www.ncbi.nlm.nih.gov/pubmed/30628672
http://dx.doi.org/10.3892/mmr.2019.9834
_version_ 1783398058678026240
author Yu, Jun
Chen, Binghai
Zheng, Bo
Qiao, Chen
Chen, Xia
Yan, Yidan
Luan, Xiaojin
Xie, Bing
Liu, Jiajia
Shen, Cong
He, Zeyu
Hu, Xing
Liu, Mingxi
Li, Hong
Shao, Qixiang
Fang, Jie
author_facet Yu, Jun
Chen, Binghai
Zheng, Bo
Qiao, Chen
Chen, Xia
Yan, Yidan
Luan, Xiaojin
Xie, Bing
Liu, Jiajia
Shen, Cong
He, Zeyu
Hu, Xing
Liu, Mingxi
Li, Hong
Shao, Qixiang
Fang, Jie
author_sort Yu, Jun
collection PubMed
description Germ cell maturation is essential for spermatogenesis and testis homeostasis. ATP synthase serves significant roles in energy storage in germ cell survival and is catalyzed by alterations in the mitochondrial membrane proton concentration. The intrinsic cellular mechanisms governing stem cell maturation remain largely unknown. In the present study, in vivo RNA interference (RNAi) screening of major ATP synthase subunits was performed, and the function of ATP synthase for male fertility and spermatogenesis in Drosophila was explored. A Upstream Activation Sequence/Gal4 transcription factor system was used to knock down gene expression in specific cell types, and immunofluorescence staining was conducted to assess the roles of ATP synthase subunits in Drosophila testes. It was identified that knockdown of ATP synthase resulted in male infertility and abnormal spermatogenesis in Drosophila testes. In addition, knockdown of the ATP synthase β subunit in germ cells resulted in defects in male infertility and germ cell maturation, while the hub and cyst cell populations were maintained. Other major ATP synthase subunits were also examined and similar phenotypes in Drosophila testes were identified. Taken together, the data from the present study revealed that ATP synthase serves important roles for male fertility during spermatogenesis by regulating germ cell maturation in Drosophila testes.
format Online
Article
Text
id pubmed-6390039
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-63900392019-03-07 ATP synthase is required for male fertility and germ cell maturation in Drosophila testes Yu, Jun Chen, Binghai Zheng, Bo Qiao, Chen Chen, Xia Yan, Yidan Luan, Xiaojin Xie, Bing Liu, Jiajia Shen, Cong He, Zeyu Hu, Xing Liu, Mingxi Li, Hong Shao, Qixiang Fang, Jie Mol Med Rep Articles Germ cell maturation is essential for spermatogenesis and testis homeostasis. ATP synthase serves significant roles in energy storage in germ cell survival and is catalyzed by alterations in the mitochondrial membrane proton concentration. The intrinsic cellular mechanisms governing stem cell maturation remain largely unknown. In the present study, in vivo RNA interference (RNAi) screening of major ATP synthase subunits was performed, and the function of ATP synthase for male fertility and spermatogenesis in Drosophila was explored. A Upstream Activation Sequence/Gal4 transcription factor system was used to knock down gene expression in specific cell types, and immunofluorescence staining was conducted to assess the roles of ATP synthase subunits in Drosophila testes. It was identified that knockdown of ATP synthase resulted in male infertility and abnormal spermatogenesis in Drosophila testes. In addition, knockdown of the ATP synthase β subunit in germ cells resulted in defects in male infertility and germ cell maturation, while the hub and cyst cell populations were maintained. Other major ATP synthase subunits were also examined and similar phenotypes in Drosophila testes were identified. Taken together, the data from the present study revealed that ATP synthase serves important roles for male fertility during spermatogenesis by regulating germ cell maturation in Drosophila testes. D.A. Spandidos 2019-03 2019-01-08 /pmc/articles/PMC6390039/ /pubmed/30628672 http://dx.doi.org/10.3892/mmr.2019.9834 Text en Copyright: © Yu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yu, Jun
Chen, Binghai
Zheng, Bo
Qiao, Chen
Chen, Xia
Yan, Yidan
Luan, Xiaojin
Xie, Bing
Liu, Jiajia
Shen, Cong
He, Zeyu
Hu, Xing
Liu, Mingxi
Li, Hong
Shao, Qixiang
Fang, Jie
ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title_full ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title_fullStr ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title_full_unstemmed ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title_short ATP synthase is required for male fertility and germ cell maturation in Drosophila testes
title_sort atp synthase is required for male fertility and germ cell maturation in drosophila testes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390039/
https://www.ncbi.nlm.nih.gov/pubmed/30628672
http://dx.doi.org/10.3892/mmr.2019.9834
work_keys_str_mv AT yujun atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT chenbinghai atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT zhengbo atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT qiaochen atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT chenxia atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT yanyidan atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT luanxiaojin atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT xiebing atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT liujiajia atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT shencong atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT hezeyu atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT huxing atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT liumingxi atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT lihong atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT shaoqixiang atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes
AT fangjie atpsynthaseisrequiredformalefertilityandgermcellmaturationindrosophilatestes