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Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice
The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390040/ https://www.ncbi.nlm.nih.gov/pubmed/30664217 http://dx.doi.org/10.3892/mmr.2019.9862 |
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author | Li, Li Xu, Xiaoyi Wu, Leilei Zhu, Haicheng He, Zhipeng Zhang, Bo Chi, Yanjun Song, Gaochen |
author_facet | Li, Li Xu, Xiaoyi Wu, Leilei Zhu, Haicheng He, Zhipeng Zhang, Bo Chi, Yanjun Song, Gaochen |
author_sort | Li, Li |
collection | PubMed |
description | The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was ~1 g (~7-10 days after inoculation). The sera proteins from each group were then collected and subjected to two-dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA-binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma-bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma-bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling. |
format | Online Article Text |
id | pubmed-6390040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63900402019-03-07 Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice Li, Li Xu, Xiaoyi Wu, Leilei Zhu, Haicheng He, Zhipeng Zhang, Bo Chi, Yanjun Song, Gaochen Mol Med Rep Articles The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was ~1 g (~7-10 days after inoculation). The sera proteins from each group were then collected and subjected to two-dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA-binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma-bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma-bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling. D.A. Spandidos 2019-03 2019-01-15 /pmc/articles/PMC6390040/ /pubmed/30664217 http://dx.doi.org/10.3892/mmr.2019.9862 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Li Xu, Xiaoyi Wu, Leilei Zhu, Haicheng He, Zhipeng Zhang, Bo Chi, Yanjun Song, Gaochen Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title | Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title_full | Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title_fullStr | Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title_full_unstemmed | Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title_short | Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice |
title_sort | scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma h22-bearing mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390040/ https://www.ncbi.nlm.nih.gov/pubmed/30664217 http://dx.doi.org/10.3892/mmr.2019.9862 |
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