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Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis

Dendropanax morbifera (D. morbifera), known as Dendro, means ‘omnipotent drug’ (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti-inflammatory effects and underlying mec...

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Autores principales: Choo, Gang-Sik, Lim, Dong-Pyo, Kim, Sae-Man, Yoo, Eun-Seon, Kim, Sung-Hyun, Kim, Chang-Hyun, Woo, Joong-Seok, Kim, Hyeong-Jin, Jung, Ji-Youn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390048/
https://www.ncbi.nlm.nih.gov/pubmed/30747232
http://dx.doi.org/10.3892/mmr.2019.9887
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author Choo, Gang-Sik
Lim, Dong-Pyo
Kim, Sae-Man
Yoo, Eun-Seon
Kim, Sung-Hyun
Kim, Chang-Hyun
Woo, Joong-Seok
Kim, Hyeong-Jin
Jung, Ji-Youn
author_facet Choo, Gang-Sik
Lim, Dong-Pyo
Kim, Sae-Man
Yoo, Eun-Seon
Kim, Sung-Hyun
Kim, Chang-Hyun
Woo, Joong-Seok
Kim, Hyeong-Jin
Jung, Ji-Youn
author_sort Choo, Gang-Sik
collection PubMed
description Dendropanax morbifera (D. morbifera), known as Dendro, means ‘omnipotent drug’ (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti-inflammatory effects and underlying mechanism of a natural extract of D. morbifera leaves (DPL) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In the present study, the following assays and models were used: MTT assay, nitric oxide (NO) assay, western blotting, ELISA and mouse models of atopic dermatitis. DPL extract markedly reduced the production of NO, inducible NO synthase and interleukin-6, as well as the nuclear translocation of nuclear factor-κB (NF-κB). Additionally, the LPS-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2), P38 and c-Jun N-terminal kinase (JNK) was suppressed by DPL extract. Taken together, these results indicate that NF-κB, ERK1/2, P38 and JNK may be potential molecular targets of DPL extract in the LPS-induced inflammatory response. Subsequently, the present study investigated the effects of DPL extract in a 2,4-dinitrochlorobenzene-induced atopic dermatitis mouse model. Ear thickness, serum immunoglobulin E levels and histological analysis revealed that the DPL extract was effective in attenuating the inflammatory response. These results indicate that DPL extract has anti-inflammatory potential and may be developed as a botanical drug to treat atopic dermatitis.
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spelling pubmed-63900482019-03-07 Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis Choo, Gang-Sik Lim, Dong-Pyo Kim, Sae-Man Yoo, Eun-Seon Kim, Sung-Hyun Kim, Chang-Hyun Woo, Joong-Seok Kim, Hyeong-Jin Jung, Ji-Youn Mol Med Rep Articles Dendropanax morbifera (D. morbifera), known as Dendro, means ‘omnipotent drug’ (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti-inflammatory effects and underlying mechanism of a natural extract of D. morbifera leaves (DPL) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In the present study, the following assays and models were used: MTT assay, nitric oxide (NO) assay, western blotting, ELISA and mouse models of atopic dermatitis. DPL extract markedly reduced the production of NO, inducible NO synthase and interleukin-6, as well as the nuclear translocation of nuclear factor-κB (NF-κB). Additionally, the LPS-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2), P38 and c-Jun N-terminal kinase (JNK) was suppressed by DPL extract. Taken together, these results indicate that NF-κB, ERK1/2, P38 and JNK may be potential molecular targets of DPL extract in the LPS-induced inflammatory response. Subsequently, the present study investigated the effects of DPL extract in a 2,4-dinitrochlorobenzene-induced atopic dermatitis mouse model. Ear thickness, serum immunoglobulin E levels and histological analysis revealed that the DPL extract was effective in attenuating the inflammatory response. These results indicate that DPL extract has anti-inflammatory potential and may be developed as a botanical drug to treat atopic dermatitis. D.A. Spandidos 2019-03 2019-01-22 /pmc/articles/PMC6390048/ /pubmed/30747232 http://dx.doi.org/10.3892/mmr.2019.9887 Text en Copyright: © Choo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Choo, Gang-Sik
Lim, Dong-Pyo
Kim, Sae-Man
Yoo, Eun-Seon
Kim, Sung-Hyun
Kim, Chang-Hyun
Woo, Joong-Seok
Kim, Hyeong-Jin
Jung, Ji-Youn
Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title_full Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title_fullStr Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title_full_unstemmed Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title_short Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis
title_sort anti-inflammatory effects of dendropanax morbifera in lipopolysaccharide-stimulated raw264.7 macrophages and in an animal model of atopic dermatitis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390048/
https://www.ncbi.nlm.nih.gov/pubmed/30747232
http://dx.doi.org/10.3892/mmr.2019.9887
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