Cargando…
Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells
Bone morphogenetic protein 9 (BMP9) belongs to the transforming growth factor-β (TGF-β) superfamily, and has been reported to promote cancer cell proliferation and epithelial-mesenchymal transition (EMT). Cisplatin (DDP) is the first line treatment for ovarian cancer. However, initiation of EMT conf...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390058/ https://www.ncbi.nlm.nih.gov/pubmed/30628686 http://dx.doi.org/10.3892/mmr.2019.9814 |
_version_ | 1783398063119794176 |
---|---|
author | Wang, Ying Yang, Bin Zhao, Jinping Yu, Xiaohui Liu, Xing Zhang, Long Zhang, Yunjing Li, Xiaoli Zhai, Zhenhua |
author_facet | Wang, Ying Yang, Bin Zhao, Jinping Yu, Xiaohui Liu, Xing Zhang, Long Zhang, Yunjing Li, Xiaoli Zhai, Zhenhua |
author_sort | Wang, Ying |
collection | PubMed |
description | Bone morphogenetic protein 9 (BMP9) belongs to the transforming growth factor-β (TGF-β) superfamily, and has been reported to promote cancer cell proliferation and epithelial-mesenchymal transition (EMT). Cisplatin (DDP) is the first line treatment for ovarian cancer. However, initiation of EMT confers insensitivity to chemotherapy. The present study aimed to verify and examine the mechanisms underlying the effects of BMP9 on treatment with DDP for ovarian cancer. Prior to treatment with DDP, ovarian cancer cells were exposed to BMP9 for 3 days. Following this, cell viability, apoptosis rate and the extent of DNA damage were evaluated to compare the effects of DDP on BMP9-pretreated and non-pretreated ovarian cancer cells. In addition, EMT marker expression was evaluated by western blotting and immunofluorescence. The results demonstrated that BMP9 pretreatment inhibited the cytotoxicity of DDP on ovarian cancer cells. Additionally, BMP9-pretreated ovarian cancer cells had downregulated expression of the epithelial marker E-cadherin, which was accompanied by an upregulation of the mesenchymal markers N-cadherin, Snail, Slug, and Twist. Taken together, the findings of the present study indicated that BMP9 conferred resistance to DDP in ovarian cancer cells by inducing EMT. The present study provided valuable insight into the mechanisms of chemotherapy in ovarian cancer and highlighted the potential of BMP9 as a novel therapeutic target for improving cisplatin chemosensitivity. |
format | Online Article Text |
id | pubmed-6390058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63900582019-03-07 Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells Wang, Ying Yang, Bin Zhao, Jinping Yu, Xiaohui Liu, Xing Zhang, Long Zhang, Yunjing Li, Xiaoli Zhai, Zhenhua Mol Med Rep Articles Bone morphogenetic protein 9 (BMP9) belongs to the transforming growth factor-β (TGF-β) superfamily, and has been reported to promote cancer cell proliferation and epithelial-mesenchymal transition (EMT). Cisplatin (DDP) is the first line treatment for ovarian cancer. However, initiation of EMT confers insensitivity to chemotherapy. The present study aimed to verify and examine the mechanisms underlying the effects of BMP9 on treatment with DDP for ovarian cancer. Prior to treatment with DDP, ovarian cancer cells were exposed to BMP9 for 3 days. Following this, cell viability, apoptosis rate and the extent of DNA damage were evaluated to compare the effects of DDP on BMP9-pretreated and non-pretreated ovarian cancer cells. In addition, EMT marker expression was evaluated by western blotting and immunofluorescence. The results demonstrated that BMP9 pretreatment inhibited the cytotoxicity of DDP on ovarian cancer cells. Additionally, BMP9-pretreated ovarian cancer cells had downregulated expression of the epithelial marker E-cadherin, which was accompanied by an upregulation of the mesenchymal markers N-cadherin, Snail, Slug, and Twist. Taken together, the findings of the present study indicated that BMP9 conferred resistance to DDP in ovarian cancer cells by inducing EMT. The present study provided valuable insight into the mechanisms of chemotherapy in ovarian cancer and highlighted the potential of BMP9 as a novel therapeutic target for improving cisplatin chemosensitivity. D.A. Spandidos 2019-03 2019-01-03 /pmc/articles/PMC6390058/ /pubmed/30628686 http://dx.doi.org/10.3892/mmr.2019.9814 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Ying Yang, Bin Zhao, Jinping Yu, Xiaohui Liu, Xing Zhang, Long Zhang, Yunjing Li, Xiaoli Zhai, Zhenhua Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title | Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title_full | Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title_fullStr | Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title_full_unstemmed | Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title_short | Epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
title_sort | epithelial-mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390058/ https://www.ncbi.nlm.nih.gov/pubmed/30628686 http://dx.doi.org/10.3892/mmr.2019.9814 |
work_keys_str_mv | AT wangying epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT yangbin epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT zhaojinping epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT yuxiaohui epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT liuxing epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT zhanglong epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT zhangyunjing epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT lixiaoli epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells AT zhaizhenhua epithelialmesenchymaltransitioninducedbybonemorphogeneticprotein9hinderscisplatinefficacyinovariancancercells |