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Optimized protocols for γδ T cell expansion and lentiviral transduction

γδ T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically-modified γδ T cells is regarded as a promising tool for tumor treatment. However, since γδ T cells constitute a minority of T cells, their la...

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Detalles Bibliográficos
Autores principales: Wang, Rui-Ning, Wen, Qian, He, Wen-Ting, Yang, Jia-Hui, Zhou, Chao-Ying, Xiong, Wen-Jing, Ma, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390064/
https://www.ncbi.nlm.nih.gov/pubmed/30628681
http://dx.doi.org/10.3892/mmr.2019.9831
Descripción
Sumario:γδ T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically-modified γδ T cells is regarded as a promising tool for tumor treatment. However, since γδ T cells constitute a minority of T cells, their large-scale expansion is difficult to realize in an efficient and cost-effective manner. In the present study, based on previous studies, culture protocols for γδ T cells were tested using different combinations of isopentenyl pyrophosphate and interleukin 2 in order to satisfy different experimental purposes. One protocol was demonstrated to be the most suitable for lentiviral transduction. These results greatly reinforce the promising prospects of using γδ T cells in basic research and for clinical applications.