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Blubber transcriptome responses to repeated ACTH administration in a marine mammal

Chronic physiological stress impacts animal fitness by catabolizing metabolic stores and suppressing reproduction. This can be especially deleterious for capital breeding carnivores such as marine mammals, with potential for ecosystem-wide effects. However, the impacts and indicators of chronic stre...

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Autores principales: Deyarmin, Jared S., McCormley, Molly C., Champagne, Cory D., Stephan, Alicia P., Busqueta, Laura Pujade, Crocker, Daniel E., Houser, Dorian S., Khudyakov, Jane I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390094/
https://www.ncbi.nlm.nih.gov/pubmed/30804370
http://dx.doi.org/10.1038/s41598-019-39089-2
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author Deyarmin, Jared S.
McCormley, Molly C.
Champagne, Cory D.
Stephan, Alicia P.
Busqueta, Laura Pujade
Crocker, Daniel E.
Houser, Dorian S.
Khudyakov, Jane I.
author_facet Deyarmin, Jared S.
McCormley, Molly C.
Champagne, Cory D.
Stephan, Alicia P.
Busqueta, Laura Pujade
Crocker, Daniel E.
Houser, Dorian S.
Khudyakov, Jane I.
author_sort Deyarmin, Jared S.
collection PubMed
description Chronic physiological stress impacts animal fitness by catabolizing metabolic stores and suppressing reproduction. This can be especially deleterious for capital breeding carnivores such as marine mammals, with potential for ecosystem-wide effects. However, the impacts and indicators of chronic stress in animals are currently poorly understood. To identify downstream mediators of repeated stress responses in marine mammals, we administered adrenocorticotropic hormone (ACTH) once daily for four days to free-ranging juvenile northern elephant seals (Mirounga angustirostris) to stimulate endogenous corticosteroid release, and compared blubber tissue transcriptome responses to the first and fourth ACTH administrations. Gene expression profiles were distinct between blubber responses to single and repeated ACTH administration, despite similarities in circulating cortisol profiles. We identified 61 and 12 genes that were differentially expressed (DEGs) in response to the first ACTH and fourth administrations, respectively, 24 DEGs between the first and fourth pre-ACTH samples, and 12 DEGs between ACTH response samples from the first and fourth days. Annotated DEGs were associated with functions in redox and lipid homeostasis, suggesting potential negative impacts of repeated stress on capital breeding, diving mammals. DEGs identified in this study are potential markers of repeated stress in marine mammals, which may not be detectable by endocrine profiles alone.
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spelling pubmed-63900942019-02-28 Blubber transcriptome responses to repeated ACTH administration in a marine mammal Deyarmin, Jared S. McCormley, Molly C. Champagne, Cory D. Stephan, Alicia P. Busqueta, Laura Pujade Crocker, Daniel E. Houser, Dorian S. Khudyakov, Jane I. Sci Rep Article Chronic physiological stress impacts animal fitness by catabolizing metabolic stores and suppressing reproduction. This can be especially deleterious for capital breeding carnivores such as marine mammals, with potential for ecosystem-wide effects. However, the impacts and indicators of chronic stress in animals are currently poorly understood. To identify downstream mediators of repeated stress responses in marine mammals, we administered adrenocorticotropic hormone (ACTH) once daily for four days to free-ranging juvenile northern elephant seals (Mirounga angustirostris) to stimulate endogenous corticosteroid release, and compared blubber tissue transcriptome responses to the first and fourth ACTH administrations. Gene expression profiles were distinct between blubber responses to single and repeated ACTH administration, despite similarities in circulating cortisol profiles. We identified 61 and 12 genes that were differentially expressed (DEGs) in response to the first ACTH and fourth administrations, respectively, 24 DEGs between the first and fourth pre-ACTH samples, and 12 DEGs between ACTH response samples from the first and fourth days. Annotated DEGs were associated with functions in redox and lipid homeostasis, suggesting potential negative impacts of repeated stress on capital breeding, diving mammals. DEGs identified in this study are potential markers of repeated stress in marine mammals, which may not be detectable by endocrine profiles alone. Nature Publishing Group UK 2019-02-25 /pmc/articles/PMC6390094/ /pubmed/30804370 http://dx.doi.org/10.1038/s41598-019-39089-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Deyarmin, Jared S.
McCormley, Molly C.
Champagne, Cory D.
Stephan, Alicia P.
Busqueta, Laura Pujade
Crocker, Daniel E.
Houser, Dorian S.
Khudyakov, Jane I.
Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title_full Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title_fullStr Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title_full_unstemmed Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title_short Blubber transcriptome responses to repeated ACTH administration in a marine mammal
title_sort blubber transcriptome responses to repeated acth administration in a marine mammal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390094/
https://www.ncbi.nlm.nih.gov/pubmed/30804370
http://dx.doi.org/10.1038/s41598-019-39089-2
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