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Protective effect of oxytocin on LPS-induced acute lung injury in mice

Oxytocin (OT), a neurohypophyseal hormone synthesized in the paraventricular and supraoptic nuclei of the hypothalamus, has been reported to have an anti- inflammatory effect. However, its role in acute lung injury (ALI) has never been investigated. The aim of this study was to explore the therapeut...

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Autores principales: An, Xiaona, Sun, Xiaotong, Hou, Yonghao, Yang, Xiaomei, Chen, Hongli, Zhang, Peng, Wu, Jianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391417/
https://www.ncbi.nlm.nih.gov/pubmed/30808956
http://dx.doi.org/10.1038/s41598-019-39349-1
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author An, Xiaona
Sun, Xiaotong
Hou, Yonghao
Yang, Xiaomei
Chen, Hongli
Zhang, Peng
Wu, Jianbo
author_facet An, Xiaona
Sun, Xiaotong
Hou, Yonghao
Yang, Xiaomei
Chen, Hongli
Zhang, Peng
Wu, Jianbo
author_sort An, Xiaona
collection PubMed
description Oxytocin (OT), a neurohypophyseal hormone synthesized in the paraventricular and supraoptic nuclei of the hypothalamus, has been reported to have an anti- inflammatory effect. However, its role in acute lung injury (ALI) has never been investigated. The aim of this study was to explore the therapeutic effects and potential mechanism action of OT on lipopolysaccharide (LPS)-induced ALI. Mice were treated with OT 30 min before the intraperitoneal injection of LPS. After 2 h, the effects of OT on lung histopathological changes, lung wet/dry (W/D) ratio, myeloperoxidase (MPO) activity, levels of inflammatory cytokines in the bronchoalveolar lavage fluid (BALF), and expression of inflammation proteins were detected. The results showed that OT significantly reduced LPS-induced pathological injury, W/D ratio, MPO activity, and the levels of interleukin (IL)-1β, IL-18 and IL-6. Further, OT also inhibited LPS-induced Toll-like receptor 4 expression and NLR family pyrin domain containing 3 inflammasome activation. OT receptor antagonist (L-368,899) was given 90 min before injecting OT to further demonstrate the role of OT in LPS-induced ALI. The results showed OT could not alleviate the aforementioned inflammatory reactions after administering L-368,899. In conclusion, the present results indicated that OT could reduce inflammatory responses of LPS-induced ALI.
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spelling pubmed-63914172019-02-28 Protective effect of oxytocin on LPS-induced acute lung injury in mice An, Xiaona Sun, Xiaotong Hou, Yonghao Yang, Xiaomei Chen, Hongli Zhang, Peng Wu, Jianbo Sci Rep Article Oxytocin (OT), a neurohypophyseal hormone synthesized in the paraventricular and supraoptic nuclei of the hypothalamus, has been reported to have an anti- inflammatory effect. However, its role in acute lung injury (ALI) has never been investigated. The aim of this study was to explore the therapeutic effects and potential mechanism action of OT on lipopolysaccharide (LPS)-induced ALI. Mice were treated with OT 30 min before the intraperitoneal injection of LPS. After 2 h, the effects of OT on lung histopathological changes, lung wet/dry (W/D) ratio, myeloperoxidase (MPO) activity, levels of inflammatory cytokines in the bronchoalveolar lavage fluid (BALF), and expression of inflammation proteins were detected. The results showed that OT significantly reduced LPS-induced pathological injury, W/D ratio, MPO activity, and the levels of interleukin (IL)-1β, IL-18 and IL-6. Further, OT also inhibited LPS-induced Toll-like receptor 4 expression and NLR family pyrin domain containing 3 inflammasome activation. OT receptor antagonist (L-368,899) was given 90 min before injecting OT to further demonstrate the role of OT in LPS-induced ALI. The results showed OT could not alleviate the aforementioned inflammatory reactions after administering L-368,899. In conclusion, the present results indicated that OT could reduce inflammatory responses of LPS-induced ALI. Nature Publishing Group UK 2019-02-26 /pmc/articles/PMC6391417/ /pubmed/30808956 http://dx.doi.org/10.1038/s41598-019-39349-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
An, Xiaona
Sun, Xiaotong
Hou, Yonghao
Yang, Xiaomei
Chen, Hongli
Zhang, Peng
Wu, Jianbo
Protective effect of oxytocin on LPS-induced acute lung injury in mice
title Protective effect of oxytocin on LPS-induced acute lung injury in mice
title_full Protective effect of oxytocin on LPS-induced acute lung injury in mice
title_fullStr Protective effect of oxytocin on LPS-induced acute lung injury in mice
title_full_unstemmed Protective effect of oxytocin on LPS-induced acute lung injury in mice
title_short Protective effect of oxytocin on LPS-induced acute lung injury in mice
title_sort protective effect of oxytocin on lps-induced acute lung injury in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391417/
https://www.ncbi.nlm.nih.gov/pubmed/30808956
http://dx.doi.org/10.1038/s41598-019-39349-1
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