Enhanced recovery of low concentration protein and peptide solutions on ultra-low binding microplates

SiO(2) Medical Products (SIO) developed PureWARE™ Ultra-Low Binding (ULB) plasma-treated microplates with the combined benefits of enhanced protein recovery and reduced extractables. This study demonstrates enhanced protein recoveries, but at ten-times lower protein concentration, or 0.1 nM, compare...

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Detalles Bibliográficos
Autores principales: Weikart, Christopher M, Breeland, Adam P, Taha, Ahmad H, Maurer, Brian R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391626/
https://www.ncbi.nlm.nih.gov/pubmed/30820347
http://dx.doi.org/10.4155/fsoa-2018-0099
Descripción
Sumario:SiO(2) Medical Products (SIO) developed PureWARE™ Ultra-Low Binding (ULB) plasma-treated microplates with the combined benefits of enhanced protein recovery and reduced extractables. This study demonstrates enhanced protein recoveries, but at ten-times lower protein concentration, or 0.1 nM, compared with a prior study. In addition, no significant effect on enhanced protein recovery of plasma-treated microplates was observed in a long-term stability study carried out for 26 months under ambient storage conditions. Furthermore, recovery of three different peptide solutions, in the concentration range of 1.5–12 nM, was also shown to be enhanced on plasma-treated microplates relative to standard polypropylene microplates.

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