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Jaw osteosarcoma models in mice: first description

BACKGROUND: Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS. METHODS: Syngenic...

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Autores principales: Bertin, Hélios, Guilho, Romain, Brion, Régis, Amiaud, Jérôme, Battaglia, Séverine, Moreau, Anne, Brouchet-Gomez, Anne, Longis, Julie, Piot, Benoit, Heymann, Dominique, Corre, Pierre, Rédini, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391788/
https://www.ncbi.nlm.nih.gov/pubmed/30813941
http://dx.doi.org/10.1186/s12967-019-1807-5
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author Bertin, Hélios
Guilho, Romain
Brion, Régis
Amiaud, Jérôme
Battaglia, Séverine
Moreau, Anne
Brouchet-Gomez, Anne
Longis, Julie
Piot, Benoit
Heymann, Dominique
Corre, Pierre
Rédini, Françoise
author_facet Bertin, Hélios
Guilho, Romain
Brion, Régis
Amiaud, Jérôme
Battaglia, Séverine
Moreau, Anne
Brouchet-Gomez, Anne
Longis, Julie
Piot, Benoit
Heymann, Dominique
Corre, Pierre
Rédini, Françoise
author_sort Bertin, Hélios
collection PubMed
description BACKGROUND: Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS. METHODS: Syngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread. RESULTS: Homogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 10(6) and 0.50 × 10(6) tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models. CONCLUSION: We describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1807-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-63917882019-03-11 Jaw osteosarcoma models in mice: first description Bertin, Hélios Guilho, Romain Brion, Régis Amiaud, Jérôme Battaglia, Séverine Moreau, Anne Brouchet-Gomez, Anne Longis, Julie Piot, Benoit Heymann, Dominique Corre, Pierre Rédini, Françoise J Transl Med Research BACKGROUND: Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS. METHODS: Syngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread. RESULTS: Homogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 10(6) and 0.50 × 10(6) tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models. CONCLUSION: We describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1807-5) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-27 /pmc/articles/PMC6391788/ /pubmed/30813941 http://dx.doi.org/10.1186/s12967-019-1807-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Bertin, Hélios
Guilho, Romain
Brion, Régis
Amiaud, Jérôme
Battaglia, Séverine
Moreau, Anne
Brouchet-Gomez, Anne
Longis, Julie
Piot, Benoit
Heymann, Dominique
Corre, Pierre
Rédini, Françoise
Jaw osteosarcoma models in mice: first description
title Jaw osteosarcoma models in mice: first description
title_full Jaw osteosarcoma models in mice: first description
title_fullStr Jaw osteosarcoma models in mice: first description
title_full_unstemmed Jaw osteosarcoma models in mice: first description
title_short Jaw osteosarcoma models in mice: first description
title_sort jaw osteosarcoma models in mice: first description
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391788/
https://www.ncbi.nlm.nih.gov/pubmed/30813941
http://dx.doi.org/10.1186/s12967-019-1807-5
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