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Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study

After a 12-week feeding trial, the glucose tolerance test was performed in Megalobrama amblycephala to evaluate the effects of metformin on the metabolic responses of glycolipids. Plasma insulin peaked at 2 h, then decreased to the basal value at 8–12 h post-injection. Plasma triglyceride levels and...

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Autores principales: Xu, Chao, Li, Xiang-Fei, Tian, Hong-Yan, Shi, Hua-Juan, Zhang, Ding-Dong, Abasubong, Kenneth Prudence, Liu, Wen-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391905/
https://www.ncbi.nlm.nih.gov/pubmed/30703066
http://dx.doi.org/10.1530/EC-18-0517
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author Xu, Chao
Li, Xiang-Fei
Tian, Hong-Yan
Shi, Hua-Juan
Zhang, Ding-Dong
Abasubong, Kenneth Prudence
Liu, Wen-Bin
author_facet Xu, Chao
Li, Xiang-Fei
Tian, Hong-Yan
Shi, Hua-Juan
Zhang, Ding-Dong
Abasubong, Kenneth Prudence
Liu, Wen-Bin
author_sort Xu, Chao
collection PubMed
description After a 12-week feeding trial, the glucose tolerance test was performed in Megalobrama amblycephala to evaluate the effects of metformin on the metabolic responses of glycolipids. Plasma insulin peaked at 2 h, then decreased to the basal value at 8–12 h post-injection. Plasma triglyceride levels and liver glycogen contents of the control group was decreased significantly during the first 2 and 1 h, respectively. Then, they returned to basal values at 12 h. During the whole sampling period, the high-carbohydrate groups had significantly higher levels of plasma metabolites and liver glycogen than those of the control group, and metformin supplementation enhanced these changes (except insulin levels). Glucose administration lowered the transcriptions of ampk α1, ampk α2, pepck, g6pase, fbpase, cpt IA and aco, the phosphorylation of Ampk α and the activities of the gluconeogenic enzymes during the first 2–4 h, while the opposite was true of glut 2, gs, gk, pk, accα and fas. High-carbohydrate diets significantly increased the transcriptions of ampk α1, ampk α2, glut 2, gs, gk, pk, accα and fas, the phosphorylation of Ampk α and the activities of the glycolytic enzymes during the whole sampling period, while the opposite was true for the remaining indicators. Furthermore, metformin significantly upregulated the aforementioned indicators (except accα and fas) and the transcriptions of cpt IA and aco. Overall, metformin benefits the glucose homeostasis of Megalobrama amblycephala fed high-carbohydrate diets through the activation of Ampk and the stimulation of glycolysis, glycogenesis and fatty acid oxidation, while depressing gluconeogenesis and lipogenesis.
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spelling pubmed-63919052019-03-05 Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study Xu, Chao Li, Xiang-Fei Tian, Hong-Yan Shi, Hua-Juan Zhang, Ding-Dong Abasubong, Kenneth Prudence Liu, Wen-Bin Endocr Connect Research After a 12-week feeding trial, the glucose tolerance test was performed in Megalobrama amblycephala to evaluate the effects of metformin on the metabolic responses of glycolipids. Plasma insulin peaked at 2 h, then decreased to the basal value at 8–12 h post-injection. Plasma triglyceride levels and liver glycogen contents of the control group was decreased significantly during the first 2 and 1 h, respectively. Then, they returned to basal values at 12 h. During the whole sampling period, the high-carbohydrate groups had significantly higher levels of plasma metabolites and liver glycogen than those of the control group, and metformin supplementation enhanced these changes (except insulin levels). Glucose administration lowered the transcriptions of ampk α1, ampk α2, pepck, g6pase, fbpase, cpt IA and aco, the phosphorylation of Ampk α and the activities of the gluconeogenic enzymes during the first 2–4 h, while the opposite was true of glut 2, gs, gk, pk, accα and fas. High-carbohydrate diets significantly increased the transcriptions of ampk α1, ampk α2, glut 2, gs, gk, pk, accα and fas, the phosphorylation of Ampk α and the activities of the glycolytic enzymes during the whole sampling period, while the opposite was true for the remaining indicators. Furthermore, metformin significantly upregulated the aforementioned indicators (except accα and fas) and the transcriptions of cpt IA and aco. Overall, metformin benefits the glucose homeostasis of Megalobrama amblycephala fed high-carbohydrate diets through the activation of Ampk and the stimulation of glycolysis, glycogenesis and fatty acid oxidation, while depressing gluconeogenesis and lipogenesis. Bioscientifica Ltd 2019-01-30 /pmc/articles/PMC6391905/ /pubmed/30703066 http://dx.doi.org/10.1530/EC-18-0517 Text en © 2019 The authors http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (http://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research
Xu, Chao
Li, Xiang-Fei
Tian, Hong-Yan
Shi, Hua-Juan
Zhang, Ding-Dong
Abasubong, Kenneth Prudence
Liu, Wen-Bin
Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title_full Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title_fullStr Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title_full_unstemmed Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title_short Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
title_sort metformin improves the glucose homeostasis of wuchang bream fed high-carbohydrate diets: a dynamic study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391905/
https://www.ncbi.nlm.nih.gov/pubmed/30703066
http://dx.doi.org/10.1530/EC-18-0517
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