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Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3

The quest for novel natural‐like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates by proteins modulates a number of important biological processes. These molec...

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Detalles Bibliográficos
Autores principales: Rahkila, Jani, Ekholm, Filip S., Ardá, Ana, Delgado, Sandra, Savolainen, Johannes, Jiménez‐Barbero, Jesús, Leino, Reko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391940/
https://www.ncbi.nlm.nih.gov/pubmed/30499163
http://dx.doi.org/10.1002/cbic.201800423
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author Rahkila, Jani
Ekholm, Filip S.
Ardá, Ana
Delgado, Sandra
Savolainen, Johannes
Jiménez‐Barbero, Jesús
Leino, Reko
author_facet Rahkila, Jani
Ekholm, Filip S.
Ardá, Ana
Delgado, Sandra
Savolainen, Johannes
Jiménez‐Barbero, Jesús
Leino, Reko
author_sort Rahkila, Jani
collection PubMed
description The quest for novel natural‐like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates by proteins modulates a number of important biological processes. These molecular recognition events are, however, difficult to study by the use of naturally occurring oligosaccharides and polysaccharides owing to their intrinsic structural heterogeneity and to the many technical difficulties encountered during the isolation of sufficient quantities of pure material for detailed structural and biological studies. Therefore, the construction of homogenous biomolecular probes that can mimic both the biophysical properties of polysaccharide backbones and the properties of bioactive oligosaccharide fragments are highly sought after. Herein, synthetic methodology for the construction of well‐defined bioconjugates consisting of biologically relevant disaccharide fragments grafted onto a dextran backbone is presented, and a preliminary NMR spectroscopy study of their interactions with galectin‐3 as a model lectin is conducted.
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spelling pubmed-63919402019-03-07 Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3 Rahkila, Jani Ekholm, Filip S. Ardá, Ana Delgado, Sandra Savolainen, Johannes Jiménez‐Barbero, Jesús Leino, Reko Chembiochem Full Papers The quest for novel natural‐like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates by proteins modulates a number of important biological processes. These molecular recognition events are, however, difficult to study by the use of naturally occurring oligosaccharides and polysaccharides owing to their intrinsic structural heterogeneity and to the many technical difficulties encountered during the isolation of sufficient quantities of pure material for detailed structural and biological studies. Therefore, the construction of homogenous biomolecular probes that can mimic both the biophysical properties of polysaccharide backbones and the properties of bioactive oligosaccharide fragments are highly sought after. Herein, synthetic methodology for the construction of well‐defined bioconjugates consisting of biologically relevant disaccharide fragments grafted onto a dextran backbone is presented, and a preliminary NMR spectroscopy study of their interactions with galectin‐3 as a model lectin is conducted. John Wiley and Sons Inc. 2018-11-30 2019-01-18 /pmc/articles/PMC6391940/ /pubmed/30499163 http://dx.doi.org/10.1002/cbic.201800423 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Full Papers
Rahkila, Jani
Ekholm, Filip S.
Ardá, Ana
Delgado, Sandra
Savolainen, Johannes
Jiménez‐Barbero, Jesús
Leino, Reko
Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title_full Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title_fullStr Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title_full_unstemmed Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title_short Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
title_sort novel dextran‐supported biological probes decorated with disaccharide entities for investigating the carbohydrate–protein interactions of gal‐3
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391940/
https://www.ncbi.nlm.nih.gov/pubmed/30499163
http://dx.doi.org/10.1002/cbic.201800423
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