A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity w...

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Autores principales: Sundukova, Mayya, Prifti, Efthymia, Bucci, Annalisa, Kirillova, Kseniia, Serrao, Joana, Reymond, Luc, Umebayashi, Miwa, Hovius, Ruud, Riezman, Howard, Johnsson, Kai, Heppenstall, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391943/
https://www.ncbi.nlm.nih.gov/pubmed/30569539
http://dx.doi.org/10.1002/anie.201812967
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author Sundukova, Mayya
Prifti, Efthymia
Bucci, Annalisa
Kirillova, Kseniia
Serrao, Joana
Reymond, Luc
Umebayashi, Miwa
Hovius, Ruud
Riezman, Howard
Johnsson, Kai
Heppenstall, Paul A.
author_facet Sundukova, Mayya
Prifti, Efthymia
Bucci, Annalisa
Kirillova, Kseniia
Serrao, Joana
Reymond, Luc
Umebayashi, Miwa
Hovius, Ruud
Riezman, Howard
Johnsson, Kai
Heppenstall, Paul A.
author_sort Sundukova, Mayya
collection PubMed
description Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons.
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spelling pubmed-63919432019-03-07 A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons Sundukova, Mayya Prifti, Efthymia Bucci, Annalisa Kirillova, Kseniia Serrao, Joana Reymond, Luc Umebayashi, Miwa Hovius, Ruud Riezman, Howard Johnsson, Kai Heppenstall, Paul A. Angew Chem Int Ed Engl Communications Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons. John Wiley and Sons Inc. 2019-01-25 2019-02-18 /pmc/articles/PMC6391943/ /pubmed/30569539 http://dx.doi.org/10.1002/anie.201812967 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Sundukova, Mayya
Prifti, Efthymia
Bucci, Annalisa
Kirillova, Kseniia
Serrao, Joana
Reymond, Luc
Umebayashi, Miwa
Hovius, Ruud
Riezman, Howard
Johnsson, Kai
Heppenstall, Paul A.
A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title_full A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title_fullStr A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title_full_unstemmed A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title_short A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
title_sort chemogenetic approach for the optical monitoring of voltage in neurons
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391943/
https://www.ncbi.nlm.nih.gov/pubmed/30569539
http://dx.doi.org/10.1002/anie.201812967
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