Cargando…

Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA

Since the amount of autologous bone for the treatment of bone defects is limited and harvesting might cause complications, synthetic bone substitutes such as the popular β-tricalcium phosphate (β-TCP) based Vitoss have been developed as an alternative grafting material. β-TCPs exhibit osteoconductiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Westhauser, Fabian, Karadjian, Maria, Essers, Christopher, Senger, Anne-Sophie, Hagmann, Sébastien, Schmidmaier, Gerhard, Moghaddam, Arash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392320/
https://www.ncbi.nlm.nih.gov/pubmed/30811492
http://dx.doi.org/10.1371/journal.pone.0212799
_version_ 1783398455740203008
author Westhauser, Fabian
Karadjian, Maria
Essers, Christopher
Senger, Anne-Sophie
Hagmann, Sébastien
Schmidmaier, Gerhard
Moghaddam, Arash
author_facet Westhauser, Fabian
Karadjian, Maria
Essers, Christopher
Senger, Anne-Sophie
Hagmann, Sébastien
Schmidmaier, Gerhard
Moghaddam, Arash
author_sort Westhauser, Fabian
collection PubMed
description Since the amount of autologous bone for the treatment of bone defects is limited and harvesting might cause complications, synthetic bone substitutes such as the popular β-tricalcium phosphate (β-TCP) based Vitoss have been developed as an alternative grafting material. β-TCPs exhibit osteoconductive properties, however material-initiated stimulation of osteogenic differentiation is limited. These limitations might be overcome by addition of 45S5 bioactive glass (BG) particles. This study aims to analyze the influence of BG particles in Vitoss BA (20 wt% BG particles with a size of 90–150 μm) on osteogenic properties, cell vitality and cell proliferation in direct comparison to Vitoss by evaluation of the underlying cellular mechanisms. For that purpose, Vitoss and Vitoss BA scaffolds were seeded with human mesenchymal stem cells (MSC) and underwent osteogenic differentiation in-vitro for up to 42 days. Cell vitality, proliferation, and osteogenic differentiation were monitored by quantitative gene expression analysis, determination of alkaline phosphatase activity, PrestoBlue cell viability assay, dsDNA quantification, and a fluorescence-microscopy-based live/dead-assay. It was demonstrated that BG particles decrease cell proliferation but do not have a negative impact on cell vitality. Especially the early stages of osteogenic differentiation were significantly improved in the presence of BG particles, resulting in earlier maturation of the MSC towards osteoblasts. Since most of the stimulatory effects induced by BG particles took place initially, particles exhibiting another surface-area-to-volume ratio should be considered in order to provide long-lasting stimulation.
format Online
Article
Text
id pubmed-6392320
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-63923202019-03-08 Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA Westhauser, Fabian Karadjian, Maria Essers, Christopher Senger, Anne-Sophie Hagmann, Sébastien Schmidmaier, Gerhard Moghaddam, Arash PLoS One Research Article Since the amount of autologous bone for the treatment of bone defects is limited and harvesting might cause complications, synthetic bone substitutes such as the popular β-tricalcium phosphate (β-TCP) based Vitoss have been developed as an alternative grafting material. β-TCPs exhibit osteoconductive properties, however material-initiated stimulation of osteogenic differentiation is limited. These limitations might be overcome by addition of 45S5 bioactive glass (BG) particles. This study aims to analyze the influence of BG particles in Vitoss BA (20 wt% BG particles with a size of 90–150 μm) on osteogenic properties, cell vitality and cell proliferation in direct comparison to Vitoss by evaluation of the underlying cellular mechanisms. For that purpose, Vitoss and Vitoss BA scaffolds were seeded with human mesenchymal stem cells (MSC) and underwent osteogenic differentiation in-vitro for up to 42 days. Cell vitality, proliferation, and osteogenic differentiation were monitored by quantitative gene expression analysis, determination of alkaline phosphatase activity, PrestoBlue cell viability assay, dsDNA quantification, and a fluorescence-microscopy-based live/dead-assay. It was demonstrated that BG particles decrease cell proliferation but do not have a negative impact on cell vitality. Especially the early stages of osteogenic differentiation were significantly improved in the presence of BG particles, resulting in earlier maturation of the MSC towards osteoblasts. Since most of the stimulatory effects induced by BG particles took place initially, particles exhibiting another surface-area-to-volume ratio should be considered in order to provide long-lasting stimulation. Public Library of Science 2019-02-27 /pmc/articles/PMC6392320/ /pubmed/30811492 http://dx.doi.org/10.1371/journal.pone.0212799 Text en © 2019 Westhauser et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Westhauser, Fabian
Karadjian, Maria
Essers, Christopher
Senger, Anne-Sophie
Hagmann, Sébastien
Schmidmaier, Gerhard
Moghaddam, Arash
Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title_full Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title_fullStr Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title_full_unstemmed Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title_short Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented β-TCP composite scaffold: an in-vitro comparison of Vitoss and Vitoss BA
title_sort osteogenic differentiation of mesenchymal stem cells is enhanced in a 45s5-supplemented β-tcp composite scaffold: an in-vitro comparison of vitoss and vitoss ba
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392320/
https://www.ncbi.nlm.nih.gov/pubmed/30811492
http://dx.doi.org/10.1371/journal.pone.0212799
work_keys_str_mv AT westhauserfabian osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT karadjianmaria osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT esserschristopher osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT sengerannesophie osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT hagmannsebastien osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT schmidmaiergerhard osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba
AT moghaddamarash osteogenicdifferentiationofmesenchymalstemcellsisenhancedina45s5supplementedbtcpcompositescaffoldaninvitrocomparisonofvitossandvitossba