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Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice
The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392498/ https://www.ncbi.nlm.nih.gov/pubmed/30810530 http://dx.doi.org/10.7554/eLife.43738 |
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author | Kojima, Mina L de Rooij, Dirk G Page, David C |
author_facet | Kojima, Mina L de Rooij, Dirk G Page, David C |
author_sort | Kojima, Mina L |
collection | PubMed |
description | The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we found that initiation involves the dramatic upregulation of a transcriptional network of thousands of genes whose expression is not limited to meiosis. This broad gene expression program is directly upregulated by STRA8, encoded by a germ cell-specific gene required for meiotic initiation. STRA8 binds its own promoter and those of thousands of other genes, including meiotic prophase genes, factors mediating DNA replication and the G1-S cell-cycle transition, and genes that promote the lengthy prophase unique to meiosis I. We conclude that, in mice, the robust amplification of this extraordinarily broad transcription program by a common factor triggers initiation of meiosis. |
format | Online Article Text |
id | pubmed-6392498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63924982019-03-04 Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice Kojima, Mina L de Rooij, Dirk G Page, David C eLife Chromosomes and Gene Expression The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we found that initiation involves the dramatic upregulation of a transcriptional network of thousands of genes whose expression is not limited to meiosis. This broad gene expression program is directly upregulated by STRA8, encoded by a germ cell-specific gene required for meiotic initiation. STRA8 binds its own promoter and those of thousands of other genes, including meiotic prophase genes, factors mediating DNA replication and the G1-S cell-cycle transition, and genes that promote the lengthy prophase unique to meiosis I. We conclude that, in mice, the robust amplification of this extraordinarily broad transcription program by a common factor triggers initiation of meiosis. eLife Sciences Publications, Ltd 2019-02-27 /pmc/articles/PMC6392498/ /pubmed/30810530 http://dx.doi.org/10.7554/eLife.43738 Text en © 2019, Kojima et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Kojima, Mina L de Rooij, Dirk G Page, David C Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title_full | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title_fullStr | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title_full_unstemmed | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title_short | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
title_sort | amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392498/ https://www.ncbi.nlm.nih.gov/pubmed/30810530 http://dx.doi.org/10.7554/eLife.43738 |
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