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Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid

In this work, binding characteristics of two hydrophilic nutraceutical models, namely epigallocatechin gallate (EGCG) and folic acid (FA), to sodium caseinate were studied by fluorimetry technique. EGCG‐loaded casein molecules were then converted to either re‐combined casein micelles (r‐CMs) or case...

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Autores principales: Malekhosseini, Parisa, Alami, Mehran, Khomeiri, Morteza, Esteghlal, Sara, Nekoei, Abdo‐Reza, Hosseini, Seyed Mohammad Hashem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392856/
https://www.ncbi.nlm.nih.gov/pubmed/30847130
http://dx.doi.org/10.1002/fsn3.827
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author Malekhosseini, Parisa
Alami, Mehran
Khomeiri, Morteza
Esteghlal, Sara
Nekoei, Abdo‐Reza
Hosseini, Seyed Mohammad Hashem
author_facet Malekhosseini, Parisa
Alami, Mehran
Khomeiri, Morteza
Esteghlal, Sara
Nekoei, Abdo‐Reza
Hosseini, Seyed Mohammad Hashem
author_sort Malekhosseini, Parisa
collection PubMed
description In this work, binding characteristics of two hydrophilic nutraceutical models, namely epigallocatechin gallate (EGCG) and folic acid (FA), to sodium caseinate were studied by fluorimetry technique. EGCG‐loaded casein molecules were then converted to either re‐combined casein micelles (r‐CMs) or casein nanoparticles (CNPs). Binding stoichiometry of EGCG and FA was 0.81 and 1.02, respectively. As determined by DLS technique, the average particle size of r‐CMs prepared at 0.5% concentration was 66.2 nm. Thermal treatment (74°C, 20 s) had significant (p < 0.05) influence on the particle size of nanocarriers, but not nutraceutical loading. The average size of CNPs was larger than that of r‐CMs. The encapsulation efficiency (EE) of EGCG was 85%, and its ejection from the nanocarrier was less than 3% over 21 days. Alkaline conditions resulted in higher release of EGCG than acidic conditions. r‐CMs were more effective than CNPs during the protection of EGCG against heat‐induced degradation. TEM micrographs confirmed the formation of r‐CMs.
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spelling pubmed-63928562019-03-07 Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid Malekhosseini, Parisa Alami, Mehran Khomeiri, Morteza Esteghlal, Sara Nekoei, Abdo‐Reza Hosseini, Seyed Mohammad Hashem Food Sci Nutr Original Research In this work, binding characteristics of two hydrophilic nutraceutical models, namely epigallocatechin gallate (EGCG) and folic acid (FA), to sodium caseinate were studied by fluorimetry technique. EGCG‐loaded casein molecules were then converted to either re‐combined casein micelles (r‐CMs) or casein nanoparticles (CNPs). Binding stoichiometry of EGCG and FA was 0.81 and 1.02, respectively. As determined by DLS technique, the average particle size of r‐CMs prepared at 0.5% concentration was 66.2 nm. Thermal treatment (74°C, 20 s) had significant (p < 0.05) influence on the particle size of nanocarriers, but not nutraceutical loading. The average size of CNPs was larger than that of r‐CMs. The encapsulation efficiency (EE) of EGCG was 85%, and its ejection from the nanocarrier was less than 3% over 21 days. Alkaline conditions resulted in higher release of EGCG than acidic conditions. r‐CMs were more effective than CNPs during the protection of EGCG against heat‐induced degradation. TEM micrographs confirmed the formation of r‐CMs. John Wiley and Sons Inc. 2019-01-24 /pmc/articles/PMC6392856/ /pubmed/30847130 http://dx.doi.org/10.1002/fsn3.827 Text en © 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Malekhosseini, Parisa
Alami, Mehran
Khomeiri, Morteza
Esteghlal, Sara
Nekoei, Abdo‐Reza
Hosseini, Seyed Mohammad Hashem
Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title_full Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title_fullStr Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title_full_unstemmed Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title_short Development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
title_sort development of casein‐based nanoencapsulation systems for delivery of epigallocatechin gallate and folic acid
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392856/
https://www.ncbi.nlm.nih.gov/pubmed/30847130
http://dx.doi.org/10.1002/fsn3.827
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