Optimization of cellulase production under solid‐state fermentation by a new mutant strain of Trichoderma reesei

Nowadays, the use of agricultural by‐products, as the cheap substrate for the production of value‐added products, is of high interest for the researchers and practitioners. Cellulase is a relatively expensive and a very important industrial enzyme where in this study was produced form rice by‐products under solid‐state fermentation. A new mutant of Trichoderma reesei was used for cellulase production. The effective variables were initially screened by “Plackett and Burman design.” Afterward, the main variables including moisture content, P source, incubation temperature, and incubation time were optimized by “one factor at a time design.” Finally, the resulting variables including 74% for moisture content, 2 g/L for K(2) HPO (4), 30°C of incubation temperature, and 4 days of incubation time were reported as the ultimate optimal condition for cellulase production.