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A genetically encoded near-infrared fluorescent calcium ion indicator
We report an intensiometric, near-infrared (NIR) fluorescent, genetically encoded calcium ion (Ca(2+)) indicator (GECI) with excitation and emission maxima at 678 nm and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca(2+) transients in cultured mammalian cells and brain...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393164/ https://www.ncbi.nlm.nih.gov/pubmed/30664778 http://dx.doi.org/10.1038/s41592-018-0294-6 |
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| author | Qian, Yong Piatkevich, Kiryl D. McLarney, Benedict Abdelfattah, Ahmed S. Mehta, Sohum Murdock, Mitchell H. Gottschalk, Sven Molina, Rosana S. Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E. Zhang, Jin Schreiter, Eric R. Shoham, Shy Razansky, Daniel Boyden, Edward S. Campbell, Robert E. |
| author_facet | Qian, Yong Piatkevich, Kiryl D. McLarney, Benedict Abdelfattah, Ahmed S. Mehta, Sohum Murdock, Mitchell H. Gottschalk, Sven Molina, Rosana S. Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E. Zhang, Jin Schreiter, Eric R. Shoham, Shy Razansky, Daniel Boyden, Edward S. Campbell, Robert E. |
| author_sort | Qian, Yong |
| collection | PubMed |
| description | We report an intensiometric, near-infrared (NIR) fluorescent, genetically encoded calcium ion (Ca(2+)) indicator (GECI) with excitation and emission maxima at 678 nm and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca(2+) transients in cultured mammalian cells and brain tissue with sensitivity comparable to currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca(2+) imaging in combination with other optogenetic indicators and actuators. |
| format | Online Article Text |
| id | pubmed-6393164 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-63931642019-07-21 A genetically encoded near-infrared fluorescent calcium ion indicator Qian, Yong Piatkevich, Kiryl D. McLarney, Benedict Abdelfattah, Ahmed S. Mehta, Sohum Murdock, Mitchell H. Gottschalk, Sven Molina, Rosana S. Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E. Zhang, Jin Schreiter, Eric R. Shoham, Shy Razansky, Daniel Boyden, Edward S. Campbell, Robert E. Nat Methods Article We report an intensiometric, near-infrared (NIR) fluorescent, genetically encoded calcium ion (Ca(2+)) indicator (GECI) with excitation and emission maxima at 678 nm and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca(2+) transients in cultured mammalian cells and brain tissue with sensitivity comparable to currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca(2+) imaging in combination with other optogenetic indicators and actuators. 2019-01-21 2019-02 /pmc/articles/PMC6393164/ /pubmed/30664778 http://dx.doi.org/10.1038/s41592-018-0294-6 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Qian, Yong Piatkevich, Kiryl D. McLarney, Benedict Abdelfattah, Ahmed S. Mehta, Sohum Murdock, Mitchell H. Gottschalk, Sven Molina, Rosana S. Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E. Zhang, Jin Schreiter, Eric R. Shoham, Shy Razansky, Daniel Boyden, Edward S. Campbell, Robert E. A genetically encoded near-infrared fluorescent calcium ion indicator |
| title | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_full | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_fullStr | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_full_unstemmed | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_short | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_sort | genetically encoded near-infrared fluorescent calcium ion indicator |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393164/ https://www.ncbi.nlm.nih.gov/pubmed/30664778 http://dx.doi.org/10.1038/s41592-018-0294-6 |
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