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ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2

Telomeres are nucleoprotein structures at the ends of linear chromosomes and present an essential feature for genome integrity. Vertebrate telomeres usually consist of hexameric TTAGGG repeats, however, in cells that use the alternative lengthening of telomeres (ALT) mechanism, variant repeat sequen...

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Autores principales: Bluhm, Alina, Viceconte, Nikenza, Li, Fudong, Rane, Grishma, Ritz, Sandra, Wang, Suman, Levin, Michal, Shi, Yunyu, Kappei, Dennis, Butter, Falk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393293/
https://www.ncbi.nlm.nih.gov/pubmed/30629181
http://dx.doi.org/10.1093/nar/gky1289
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author Bluhm, Alina
Viceconte, Nikenza
Li, Fudong
Rane, Grishma
Ritz, Sandra
Wang, Suman
Levin, Michal
Shi, Yunyu
Kappei, Dennis
Butter, Falk
author_facet Bluhm, Alina
Viceconte, Nikenza
Li, Fudong
Rane, Grishma
Ritz, Sandra
Wang, Suman
Levin, Michal
Shi, Yunyu
Kappei, Dennis
Butter, Falk
author_sort Bluhm, Alina
collection PubMed
description Telomeres are nucleoprotein structures at the ends of linear chromosomes and present an essential feature for genome integrity. Vertebrate telomeres usually consist of hexameric TTAGGG repeats, however, in cells that use the alternative lengthening of telomeres (ALT) mechanism, variant repeat sequences are interspersed throughout telomeres. Previously, it was shown that NR2C/F transcription factors bind to TCAGGG variant repeats and contribute to telomere maintenance in ALT cells. While specific binders to other variant repeat sequences have been lacking to date, we here identify ZBTB10 as the first TTGGGG-binding protein and demonstrate direct binding via the two zinc fingers with affinity in the nanomolar range. Concomitantly, ZBTB10 co-localizes with a subset of telomeres in ALT-positive U2OS cells and interacts with TRF2/RAP1 via the N-terminal region of TRF2. Our data establishes ZBTB10 as a novel variant repeat binding protein at ALT telomeres.
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spelling pubmed-63932932019-03-05 ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2 Bluhm, Alina Viceconte, Nikenza Li, Fudong Rane, Grishma Ritz, Sandra Wang, Suman Levin, Michal Shi, Yunyu Kappei, Dennis Butter, Falk Nucleic Acids Res Molecular Biology Telomeres are nucleoprotein structures at the ends of linear chromosomes and present an essential feature for genome integrity. Vertebrate telomeres usually consist of hexameric TTAGGG repeats, however, in cells that use the alternative lengthening of telomeres (ALT) mechanism, variant repeat sequences are interspersed throughout telomeres. Previously, it was shown that NR2C/F transcription factors bind to TCAGGG variant repeats and contribute to telomere maintenance in ALT cells. While specific binders to other variant repeat sequences have been lacking to date, we here identify ZBTB10 as the first TTGGGG-binding protein and demonstrate direct binding via the two zinc fingers with affinity in the nanomolar range. Concomitantly, ZBTB10 co-localizes with a subset of telomeres in ALT-positive U2OS cells and interacts with TRF2/RAP1 via the N-terminal region of TRF2. Our data establishes ZBTB10 as a novel variant repeat binding protein at ALT telomeres. Oxford University Press 2019-02-28 2019-01-10 /pmc/articles/PMC6393293/ /pubmed/30629181 http://dx.doi.org/10.1093/nar/gky1289 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
Bluhm, Alina
Viceconte, Nikenza
Li, Fudong
Rane, Grishma
Ritz, Sandra
Wang, Suman
Levin, Michal
Shi, Yunyu
Kappei, Dennis
Butter, Falk
ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title_full ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title_fullStr ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title_full_unstemmed ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title_short ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
title_sort zbtb10 binds the telomeric variant repeat ttgggg and interacts with trf2
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393293/
https://www.ncbi.nlm.nih.gov/pubmed/30629181
http://dx.doi.org/10.1093/nar/gky1289
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