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Long fragments achieve lower base quality in Illumina paired-end sequencing

Illumina’s technology provides high quality reads of DNA fragments with error rates below 1/1000 per base. Sequencing runs typically generate millions of reads in which the vast majority of the reads has an average error rate below 1/1000. However, some paired-end sequencing data show the presence o...

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Detalles Bibliográficos
Autores principales: Tan, Ge, Opitz, Lennart, Schlapbach, Ralph, Rehrauer, Hubert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393434/
https://www.ncbi.nlm.nih.gov/pubmed/30814542
http://dx.doi.org/10.1038/s41598-019-39076-7