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Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis

Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was develop...

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Autores principales: Chiewchankaset, Porntip, Siriwat, Wanatsanan, Suksangpanomrung, Malinee, Boonseng, Opas, Meechai, Asawin, Tanticharoen, Morakot, Kalapanulak, Saowalak, Saithong, Treenut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393550/
https://www.ncbi.nlm.nih.gov/pubmed/30814632
http://dx.doi.org/10.1038/s41598-019-39920-w
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author Chiewchankaset, Porntip
Siriwat, Wanatsanan
Suksangpanomrung, Malinee
Boonseng, Opas
Meechai, Asawin
Tanticharoen, Morakot
Kalapanulak, Saowalak
Saithong, Treenut
author_facet Chiewchankaset, Porntip
Siriwat, Wanatsanan
Suksangpanomrung, Malinee
Boonseng, Opas
Meechai, Asawin
Tanticharoen, Morakot
Kalapanulak, Saowalak
Saithong, Treenut
author_sort Chiewchankaset, Porntip
collection PubMed
description Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was developed based on the carbon assimilation pathway of cassava. Following the subcellular compartmentalization and curation to ensure full network connectivity and reflect the complexity of eukaryotic cells, cultivar specific data on sucrose uptake and biomass synthesis were input, and rMeCBM model was used to simulate storage root growth in KU50 and HN. Results showed that rMeCBM-KU50 and rMeCBM-HN models well imitated the storage root growth. The flux-sum analysis revealed that both cultivars utilized different metabolic precursors to produce energy in plastid. More carbon flux was invested in the syntheses of carbohydrates and amino acids in KU50 than in HN. Also, KU50 utilized less flux for respiration and less energy to synthesize one gram of dry storage root. These results may disclose metabolic potential of KU50 underlying its higher storage root and starch yield over HN. Moreover, sensitivity analysis indicated the robustness of rMeCBM model. The knowledge gained might be useful for identifying engineering targets for cassava yield improvement.
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spelling pubmed-63935502019-03-01 Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis Chiewchankaset, Porntip Siriwat, Wanatsanan Suksangpanomrung, Malinee Boonseng, Opas Meechai, Asawin Tanticharoen, Morakot Kalapanulak, Saowalak Saithong, Treenut Sci Rep Article Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was developed based on the carbon assimilation pathway of cassava. Following the subcellular compartmentalization and curation to ensure full network connectivity and reflect the complexity of eukaryotic cells, cultivar specific data on sucrose uptake and biomass synthesis were input, and rMeCBM model was used to simulate storage root growth in KU50 and HN. Results showed that rMeCBM-KU50 and rMeCBM-HN models well imitated the storage root growth. The flux-sum analysis revealed that both cultivars utilized different metabolic precursors to produce energy in plastid. More carbon flux was invested in the syntheses of carbohydrates and amino acids in KU50 than in HN. Also, KU50 utilized less flux for respiration and less energy to synthesize one gram of dry storage root. These results may disclose metabolic potential of KU50 underlying its higher storage root and starch yield over HN. Moreover, sensitivity analysis indicated the robustness of rMeCBM model. The knowledge gained might be useful for identifying engineering targets for cassava yield improvement. Nature Publishing Group UK 2019-02-27 /pmc/articles/PMC6393550/ /pubmed/30814632 http://dx.doi.org/10.1038/s41598-019-39920-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chiewchankaset, Porntip
Siriwat, Wanatsanan
Suksangpanomrung, Malinee
Boonseng, Opas
Meechai, Asawin
Tanticharoen, Morakot
Kalapanulak, Saowalak
Saithong, Treenut
Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title_full Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title_fullStr Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title_full_unstemmed Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title_short Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
title_sort understanding carbon utilization routes between high and low starch-producing cultivars of cassava through flux balance analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393550/
https://www.ncbi.nlm.nih.gov/pubmed/30814632
http://dx.doi.org/10.1038/s41598-019-39920-w
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