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Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis
Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was develop...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393550/ https://www.ncbi.nlm.nih.gov/pubmed/30814632 http://dx.doi.org/10.1038/s41598-019-39920-w |
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author | Chiewchankaset, Porntip Siriwat, Wanatsanan Suksangpanomrung, Malinee Boonseng, Opas Meechai, Asawin Tanticharoen, Morakot Kalapanulak, Saowalak Saithong, Treenut |
author_facet | Chiewchankaset, Porntip Siriwat, Wanatsanan Suksangpanomrung, Malinee Boonseng, Opas Meechai, Asawin Tanticharoen, Morakot Kalapanulak, Saowalak Saithong, Treenut |
author_sort | Chiewchankaset, Porntip |
collection | PubMed |
description | Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was developed based on the carbon assimilation pathway of cassava. Following the subcellular compartmentalization and curation to ensure full network connectivity and reflect the complexity of eukaryotic cells, cultivar specific data on sucrose uptake and biomass synthesis were input, and rMeCBM model was used to simulate storage root growth in KU50 and HN. Results showed that rMeCBM-KU50 and rMeCBM-HN models well imitated the storage root growth. The flux-sum analysis revealed that both cultivars utilized different metabolic precursors to produce energy in plastid. More carbon flux was invested in the syntheses of carbohydrates and amino acids in KU50 than in HN. Also, KU50 utilized less flux for respiration and less energy to synthesize one gram of dry storage root. These results may disclose metabolic potential of KU50 underlying its higher storage root and starch yield over HN. Moreover, sensitivity analysis indicated the robustness of rMeCBM model. The knowledge gained might be useful for identifying engineering targets for cassava yield improvement. |
format | Online Article Text |
id | pubmed-6393550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63935502019-03-01 Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis Chiewchankaset, Porntip Siriwat, Wanatsanan Suksangpanomrung, Malinee Boonseng, Opas Meechai, Asawin Tanticharoen, Morakot Kalapanulak, Saowalak Saithong, Treenut Sci Rep Article Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the constraint-based metabolic model of cassava storage roots, rMeCBM, was developed based on the carbon assimilation pathway of cassava. Following the subcellular compartmentalization and curation to ensure full network connectivity and reflect the complexity of eukaryotic cells, cultivar specific data on sucrose uptake and biomass synthesis were input, and rMeCBM model was used to simulate storage root growth in KU50 and HN. Results showed that rMeCBM-KU50 and rMeCBM-HN models well imitated the storage root growth. The flux-sum analysis revealed that both cultivars utilized different metabolic precursors to produce energy in plastid. More carbon flux was invested in the syntheses of carbohydrates and amino acids in KU50 than in HN. Also, KU50 utilized less flux for respiration and less energy to synthesize one gram of dry storage root. These results may disclose metabolic potential of KU50 underlying its higher storage root and starch yield over HN. Moreover, sensitivity analysis indicated the robustness of rMeCBM model. The knowledge gained might be useful for identifying engineering targets for cassava yield improvement. Nature Publishing Group UK 2019-02-27 /pmc/articles/PMC6393550/ /pubmed/30814632 http://dx.doi.org/10.1038/s41598-019-39920-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chiewchankaset, Porntip Siriwat, Wanatsanan Suksangpanomrung, Malinee Boonseng, Opas Meechai, Asawin Tanticharoen, Morakot Kalapanulak, Saowalak Saithong, Treenut Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title | Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title_full | Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title_fullStr | Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title_full_unstemmed | Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title_short | Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis |
title_sort | understanding carbon utilization routes between high and low starch-producing cultivars of cassava through flux balance analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393550/ https://www.ncbi.nlm.nih.gov/pubmed/30814632 http://dx.doi.org/10.1038/s41598-019-39920-w |
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