Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yello...

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Detalles Bibliográficos
Autores principales: Marvin, Jonathan S., Scholl, Benjamin, Wilson, Daniel E., Podgorski, Kaspar, Kazemipour, Abbas, Müller, Johannes Alexander, Schoch, Susanne, Quiroz, Francisco José Urra, Rebola, Nelson, Bao, Huan, Little, Justin P., Tkachuk, Ariana N., Cai, Edward, Hantman, Adam W., Wang, Samuel S.-H., DePiero, Victor J., Borghuis, Bart G., Chapman, Edwin R., Dietrich, Dirk, DiGregorio, David A., Fitzpatrick, David, Looger, Loren L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394230/
https://www.ncbi.nlm.nih.gov/pubmed/30377363
http://dx.doi.org/10.1038/s41592-018-0171-3
Descripción
Sumario:Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yellow emission profiles. These variants allow in vivo imaging where original iGluSnFR was too dim, can resolve glutamate transients in dendritic spines and axonal boutons, and permit kilohertz imaging.

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