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Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yello...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394230/ https://www.ncbi.nlm.nih.gov/pubmed/30377363 http://dx.doi.org/10.1038/s41592-018-0171-3 |
| _version_ | 1783398850528018432 |
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| author | Marvin, Jonathan S. Scholl, Benjamin Wilson, Daniel E. Podgorski, Kaspar Kazemipour, Abbas Müller, Johannes Alexander Schoch, Susanne Quiroz, Francisco José Urra Rebola, Nelson Bao, Huan Little, Justin P. Tkachuk, Ariana N. Cai, Edward Hantman, Adam W. Wang, Samuel S.-H. DePiero, Victor J. Borghuis, Bart G. Chapman, Edwin R. Dietrich, Dirk DiGregorio, David A. Fitzpatrick, David Looger, Loren L. |
| author_facet | Marvin, Jonathan S. Scholl, Benjamin Wilson, Daniel E. Podgorski, Kaspar Kazemipour, Abbas Müller, Johannes Alexander Schoch, Susanne Quiroz, Francisco José Urra Rebola, Nelson Bao, Huan Little, Justin P. Tkachuk, Ariana N. Cai, Edward Hantman, Adam W. Wang, Samuel S.-H. DePiero, Victor J. Borghuis, Bart G. Chapman, Edwin R. Dietrich, Dirk DiGregorio, David A. Fitzpatrick, David Looger, Loren L. |
| author_sort | Marvin, Jonathan S. |
| collection | PubMed |
| description | Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yellow emission profiles. These variants allow in vivo imaging where original iGluSnFR was too dim, can resolve glutamate transients in dendritic spines and axonal boutons, and permit kilohertz imaging. |
| format | Online Article Text |
| id | pubmed-6394230 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-63942302019-04-30 Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR Marvin, Jonathan S. Scholl, Benjamin Wilson, Daniel E. Podgorski, Kaspar Kazemipour, Abbas Müller, Johannes Alexander Schoch, Susanne Quiroz, Francisco José Urra Rebola, Nelson Bao, Huan Little, Justin P. Tkachuk, Ariana N. Cai, Edward Hantman, Adam W. Wang, Samuel S.-H. DePiero, Victor J. Borghuis, Bart G. Chapman, Edwin R. Dietrich, Dirk DiGregorio, David A. Fitzpatrick, David Looger, Loren L. Nat Methods Article Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yellow emission profiles. These variants allow in vivo imaging where original iGluSnFR was too dim, can resolve glutamate transients in dendritic spines and axonal boutons, and permit kilohertz imaging. 2018-10-30 2018-11 /pmc/articles/PMC6394230/ /pubmed/30377363 http://dx.doi.org/10.1038/s41592-018-0171-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Marvin, Jonathan S. Scholl, Benjamin Wilson, Daniel E. Podgorski, Kaspar Kazemipour, Abbas Müller, Johannes Alexander Schoch, Susanne Quiroz, Francisco José Urra Rebola, Nelson Bao, Huan Little, Justin P. Tkachuk, Ariana N. Cai, Edward Hantman, Adam W. Wang, Samuel S.-H. DePiero, Victor J. Borghuis, Bart G. Chapman, Edwin R. Dietrich, Dirk DiGregorio, David A. Fitzpatrick, David Looger, Loren L. Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title | Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title_full | Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title_fullStr | Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title_full_unstemmed | Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title_short | Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR |
| title_sort | stability, affinity and chromatic variants of the glutamate sensor iglusnfr |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394230/ https://www.ncbi.nlm.nih.gov/pubmed/30377363 http://dx.doi.org/10.1038/s41592-018-0171-3 |
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