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Emergence of visible light optical properties of L-phenylalanine aggregates
The ability of phenylalanine to form fibrillar nanostructures was demonstrated on multiple occasions, and such an oligomerization reaction could be the cause of cytotoxicity in patients with phenylketonuria. These findings were supported by claims that L-phenylalanine (Phe) fibrils have amyloid prop...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394350/ https://www.ncbi.nlm.nih.gov/pubmed/30828498 http://dx.doi.org/10.7717/peerj.6518 |
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author | Ziaunys, Mantas Smirnovas, Vytautas |
author_facet | Ziaunys, Mantas Smirnovas, Vytautas |
author_sort | Ziaunys, Mantas |
collection | PubMed |
description | The ability of phenylalanine to form fibrillar nanostructures was demonstrated on multiple occasions, and such an oligomerization reaction could be the cause of cytotoxicity in patients with phenylketonuria. These findings were supported by claims that L-phenylalanine (Phe) fibrils have amyloid properties and can be detected using thioflavin T fluorescence assay. However, a part of Phe aggregation studies reported the opposite data, suggesting no amyloid structures to be formed. Due to the contradicting reports, the amyloid nature of Phe aggregates remains uncertain. In this work we tested Phe aggregation under conditions where amyloid formation was previously reported. We show the emergence of Phe aggregates with visible light optical properties that overlap with the spectra of dyes used in amyloid fibril assays, which could lead to false-positive identifications. |
format | Online Article Text |
id | pubmed-6394350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63943502019-03-01 Emergence of visible light optical properties of L-phenylalanine aggregates Ziaunys, Mantas Smirnovas, Vytautas PeerJ Biochemistry The ability of phenylalanine to form fibrillar nanostructures was demonstrated on multiple occasions, and such an oligomerization reaction could be the cause of cytotoxicity in patients with phenylketonuria. These findings were supported by claims that L-phenylalanine (Phe) fibrils have amyloid properties and can be detected using thioflavin T fluorescence assay. However, a part of Phe aggregation studies reported the opposite data, suggesting no amyloid structures to be formed. Due to the contradicting reports, the amyloid nature of Phe aggregates remains uncertain. In this work we tested Phe aggregation under conditions where amyloid formation was previously reported. We show the emergence of Phe aggregates with visible light optical properties that overlap with the spectra of dyes used in amyloid fibril assays, which could lead to false-positive identifications. PeerJ Inc. 2019-02-25 /pmc/articles/PMC6394350/ /pubmed/30828498 http://dx.doi.org/10.7717/peerj.6518 Text en © 2019 Ziaunys and Smirnovas http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Ziaunys, Mantas Smirnovas, Vytautas Emergence of visible light optical properties of L-phenylalanine aggregates |
title | Emergence of visible light optical properties of L-phenylalanine aggregates |
title_full | Emergence of visible light optical properties of L-phenylalanine aggregates |
title_fullStr | Emergence of visible light optical properties of L-phenylalanine aggregates |
title_full_unstemmed | Emergence of visible light optical properties of L-phenylalanine aggregates |
title_short | Emergence of visible light optical properties of L-phenylalanine aggregates |
title_sort | emergence of visible light optical properties of l-phenylalanine aggregates |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394350/ https://www.ncbi.nlm.nih.gov/pubmed/30828498 http://dx.doi.org/10.7717/peerj.6518 |
work_keys_str_mv | AT ziaunysmantas emergenceofvisiblelightopticalpropertiesoflphenylalanineaggregates AT smirnovasvytautas emergenceofvisiblelightopticalpropertiesoflphenylalanineaggregates |