Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis

INTRODUCTION: Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites. OBJECTIVES: A method to keep the shape of plant tissue needs to be developed for MALDI−IMS. METHODS: The method was developed using...

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Detalles Bibliográficos
Autores principales: Nakabayashi, Ryo, Hashimoto, Kei, Toyooka, Kiminori, Saito, Kazuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394462/
https://www.ncbi.nlm.nih.gov/pubmed/30830429
http://dx.doi.org/10.1007/s11306-019-1486-5
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author Nakabayashi, Ryo
Hashimoto, Kei
Toyooka, Kiminori
Saito, Kazuki
author_facet Nakabayashi, Ryo
Hashimoto, Kei
Toyooka, Kiminori
Saito, Kazuki
author_sort Nakabayashi, Ryo
collection PubMed
description INTRODUCTION: Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites. OBJECTIVES: A method to keep the shape of plant tissue needs to be developed for MALDI−IMS. METHODS: The method was developed using transfer tape and double-sided conductive tape. RESULTS: MALDI−IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features. CONCLUSION: This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-019-1486-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-63944622019-03-15 Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis Nakabayashi, Ryo Hashimoto, Kei Toyooka, Kiminori Saito, Kazuki Metabolomics Original Article INTRODUCTION: Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites. OBJECTIVES: A method to keep the shape of plant tissue needs to be developed for MALDI−IMS. METHODS: The method was developed using transfer tape and double-sided conductive tape. RESULTS: MALDI−IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features. CONCLUSION: This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-019-1486-5) contains supplementary material, which is available to authorized users. Springer US 2019-02-14 2019 /pmc/articles/PMC6394462/ /pubmed/30830429 http://dx.doi.org/10.1007/s11306-019-1486-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Nakabayashi, Ryo
Hashimoto, Kei
Toyooka, Kiminori
Saito, Kazuki
Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title_full Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title_fullStr Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title_full_unstemmed Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title_short Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
title_sort keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in asparagus officinalis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394462/
https://www.ncbi.nlm.nih.gov/pubmed/30830429
http://dx.doi.org/10.1007/s11306-019-1486-5
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