The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis

BACKGROUND/PURPOSE: Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), as a conventional molecular technique, was utilized to analyze the diversity of oral microbiota. However, studies found that the results of PCR-DGGE were affected by the DNA isolation method. This study...

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Detalles Bibliográficos
Autores principales: Chen, Ming, Wu, Bu-Ling, Chen, Ting, Liu, Zhao, Deng, Zi-Long, Peng, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association for Dental Sciences of the Republic of China 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395153/
https://www.ncbi.nlm.nih.gov/pubmed/30894946
http://dx.doi.org/10.1016/j.jds.2015.08.002

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