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Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms

BACKGROUND/PURPOSE: The co-operative effect of exogenous dextranase (Dex) and sodium fluoride (NaF) on Streptococcus mutans monospecies biofilms is impressive. Here we investigated the effects of the combination on a mature cariogenic multispecies biofilm and analyzed the potential mechanism. MATERI...

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Autores principales: Qiu, Yuan-xin, Mao, Meng-ying, Jiang, Dan, Hong, Xiao, Yang, Ying-ming, Hu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association for Dental Sciences of the Republic of China 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395195/
https://www.ncbi.nlm.nih.gov/pubmed/30894944
http://dx.doi.org/10.1016/j.jds.2015.08.001
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author Qiu, Yuan-xin
Mao, Meng-ying
Jiang, Dan
Hong, Xiao
Yang, Ying-ming
Hu, Tao
author_facet Qiu, Yuan-xin
Mao, Meng-ying
Jiang, Dan
Hong, Xiao
Yang, Ying-ming
Hu, Tao
author_sort Qiu, Yuan-xin
collection PubMed
description BACKGROUND/PURPOSE: The co-operative effect of exogenous dextranase (Dex) and sodium fluoride (NaF) on Streptococcus mutans monospecies biofilms is impressive. Here we investigated the effects of the combination on a mature cariogenic multispecies biofilm and analyzed the potential mechanism. MATERIALS AND METHODS: A multispecies biofilm of S. mutans, Lactobacillus acidophilus, and Actinomyces viscosus was established in vitro. Dex and NaF were added separately or together. The effects of the agents on the biomass were measured. The exopolysaccharide production was determined with the scintillation counting method. The viability and morphology were evaluated using colony forming unit and confocal laser scanning microscopy, respectively. RESULTS: In general, biofilms treated with Dex and a little concentration of NaF exhibited a lower biomass, exopolysaccharide production, and viability compared with the control group (P < 0.05). Confocal laser scanning microscopy using a vital fluorescence technique showed the combination treated biofilms appeared to be loose relatively and single cells could be observed. Furthermore, the thickness and viability were also lower than either of the separate agent groups (P < 0.05). CONCLUSION: Overall, these findings reveal that a combination of 1 U/mL Dex and 80 μg/mL NaF is a promising candidate for disrupting complex cariogenic multispecies biofilms. This feature may be in that Dex loses the structure of biofilms, thereby facilitating NaF penetration and enhancing its antibacterial effects.
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spelling pubmed-63951952019-03-20 Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms Qiu, Yuan-xin Mao, Meng-ying Jiang, Dan Hong, Xiao Yang, Ying-ming Hu, Tao J Dent Sci Original Article BACKGROUND/PURPOSE: The co-operative effect of exogenous dextranase (Dex) and sodium fluoride (NaF) on Streptococcus mutans monospecies biofilms is impressive. Here we investigated the effects of the combination on a mature cariogenic multispecies biofilm and analyzed the potential mechanism. MATERIALS AND METHODS: A multispecies biofilm of S. mutans, Lactobacillus acidophilus, and Actinomyces viscosus was established in vitro. Dex and NaF were added separately or together. The effects of the agents on the biomass were measured. The exopolysaccharide production was determined with the scintillation counting method. The viability and morphology were evaluated using colony forming unit and confocal laser scanning microscopy, respectively. RESULTS: In general, biofilms treated with Dex and a little concentration of NaF exhibited a lower biomass, exopolysaccharide production, and viability compared with the control group (P < 0.05). Confocal laser scanning microscopy using a vital fluorescence technique showed the combination treated biofilms appeared to be loose relatively and single cells could be observed. Furthermore, the thickness and viability were also lower than either of the separate agent groups (P < 0.05). CONCLUSION: Overall, these findings reveal that a combination of 1 U/mL Dex and 80 μg/mL NaF is a promising candidate for disrupting complex cariogenic multispecies biofilms. This feature may be in that Dex loses the structure of biofilms, thereby facilitating NaF penetration and enhancing its antibacterial effects. Association for Dental Sciences of the Republic of China 2016-03 2015-11-21 /pmc/articles/PMC6395195/ /pubmed/30894944 http://dx.doi.org/10.1016/j.jds.2015.08.001 Text en Copyright © 2015, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. This is an open access. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Qiu, Yuan-xin
Mao, Meng-ying
Jiang, Dan
Hong, Xiao
Yang, Ying-ming
Hu, Tao
Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title_full Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title_fullStr Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title_full_unstemmed Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title_short Co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
title_sort co-operative effect of exogenous dextranase and sodium fluoride on multispecies biofilms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395195/
https://www.ncbi.nlm.nih.gov/pubmed/30894944
http://dx.doi.org/10.1016/j.jds.2015.08.001
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