Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma

Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and trig...

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Autores principales: PASCIU, Valeria, BARALLA, Elena, NIEDDU, Maria, SUCCU, Sara, PORCU, Cristian, LEONI, Giovanni G., SECHI, Pietro, BOMBOI, Giovanni C., BERLINGUER, Fiammetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395207/
https://www.ncbi.nlm.nih.gov/pubmed/30068832
http://dx.doi.org/10.1292/jvms.18-0356
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author PASCIU, Valeria
BARALLA, Elena
NIEDDU, Maria
SUCCU, Sara
PORCU, Cristian
LEONI, Giovanni G.
SECHI, Pietro
BOMBOI, Giovanni C.
BERLINGUER, Fiammetta
author_facet PASCIU, Valeria
BARALLA, Elena
NIEDDU, Maria
SUCCU, Sara
PORCU, Cristian
LEONI, Giovanni G.
SECHI, Pietro
BOMBOI, Giovanni C.
BERLINGUER, Fiammetta
author_sort PASCIU, Valeria
collection PubMed
description Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes.
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spelling pubmed-63952072019-03-06 Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma PASCIU, Valeria BARALLA, Elena NIEDDU, Maria SUCCU, Sara PORCU, Cristian LEONI, Giovanni G. SECHI, Pietro BOMBOI, Giovanni C. BERLINGUER, Fiammetta J Vet Med Sci Physiology Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes. The Japanese Society of Veterinary Science 2018-08-01 2019-02 /pmc/articles/PMC6395207/ /pubmed/30068832 http://dx.doi.org/10.1292/jvms.18-0356 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Physiology
PASCIU, Valeria
BARALLA, Elena
NIEDDU, Maria
SUCCU, Sara
PORCU, Cristian
LEONI, Giovanni G.
SECHI, Pietro
BOMBOI, Giovanni C.
BERLINGUER, Fiammetta
Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title_full Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title_fullStr Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title_full_unstemmed Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title_short Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
title_sort commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395207/
https://www.ncbi.nlm.nih.gov/pubmed/30068832
http://dx.doi.org/10.1292/jvms.18-0356
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