Cargando…

Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)

BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated os...

Descripción completa

Detalles Bibliográficos
Autores principales: Pramono, Samdharu, Pugdee, Kamolparn, Suwanprateep, Jintamai, Koontongkaew, Sittichai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association for Dental Sciences of the Republic of China 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395237/
https://www.ncbi.nlm.nih.gov/pubmed/30895008
http://dx.doi.org/10.1016/j.jds.2016.07.004
_version_ 1783399050635116544
author Pramono, Samdharu
Pugdee, Kamolparn
Suwanprateep, Jintamai
Koontongkaew, Sittichai
author_facet Pramono, Samdharu
Pugdee, Kamolparn
Suwanprateep, Jintamai
Koontongkaew, Sittichai
author_sort Pramono, Samdharu
collection PubMed
description BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated osteoblast-like cell adhesion, spreading, proliferation, and differentiation. MATERIALS AND METHODS: Osteoblast-like cells (MC3T3-E1) were cultured on sandblasted Ti disks immobilized with FN or FN-derived peptides [GRGDSP (Gly-Arg-Gly-Asp-Ser), PHSRN (Pro-His-Ser-Arg-Asn), or GRGDSP/PHSRN]. Surface topography, cell morphology, cell adhesion, cell proliferation, analysis of osteogenesis-related genes and protein expression, alkaline phosphatase, and alizarin red staining of mineralization were evaluated. RESULTS: The sandblasted Ti coated with FN or FN-derived peptides enhanced cell adhesion and cell proliferation. However, the Ti coated with FN or FN-derived peptides groups were similar in cell spreading. Osteogenic differentiation was observed in the peptide-modified Ti surface groups, compared with that of the noncoated Ti group. FN and GRGDSP/PHSRN coating enhanced the gene and protein expression of Runx2, osteocalcin, and bone sialoprotein. Alkaline phosphatase activity and matrix mineralization were also markedly enhanced in the Ti coated groups. CONCLUSION: The sandblasted Ti coated with FN or FN-derived peptides (GRGDSP/PHSRN) markedly enhance adhesion, proliferation, and differentiation of osteoblast-like cells compared with uncoated sandblasted Ti.
format Online
Article
Text
id pubmed-6395237
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Association for Dental Sciences of the Republic of China
record_format MEDLINE/PubMed
spelling pubmed-63952372019-03-20 Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) Pramono, Samdharu Pugdee, Kamolparn Suwanprateep, Jintamai Koontongkaew, Sittichai J Dent Sci Original Article BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated osteoblast-like cell adhesion, spreading, proliferation, and differentiation. MATERIALS AND METHODS: Osteoblast-like cells (MC3T3-E1) were cultured on sandblasted Ti disks immobilized with FN or FN-derived peptides [GRGDSP (Gly-Arg-Gly-Asp-Ser), PHSRN (Pro-His-Ser-Arg-Asn), or GRGDSP/PHSRN]. Surface topography, cell morphology, cell adhesion, cell proliferation, analysis of osteogenesis-related genes and protein expression, alkaline phosphatase, and alizarin red staining of mineralization were evaluated. RESULTS: The sandblasted Ti coated with FN or FN-derived peptides enhanced cell adhesion and cell proliferation. However, the Ti coated with FN or FN-derived peptides groups were similar in cell spreading. Osteogenic differentiation was observed in the peptide-modified Ti surface groups, compared with that of the noncoated Ti group. FN and GRGDSP/PHSRN coating enhanced the gene and protein expression of Runx2, osteocalcin, and bone sialoprotein. Alkaline phosphatase activity and matrix mineralization were also markedly enhanced in the Ti coated groups. CONCLUSION: The sandblasted Ti coated with FN or FN-derived peptides (GRGDSP/PHSRN) markedly enhance adhesion, proliferation, and differentiation of osteoblast-like cells compared with uncoated sandblasted Ti. Association for Dental Sciences of the Republic of China 2016-12 2016-11-09 /pmc/articles/PMC6395237/ /pubmed/30895008 http://dx.doi.org/10.1016/j.jds.2016.07.004 Text en Copyright © 2016, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Pramono, Samdharu
Pugdee, Kamolparn
Suwanprateep, Jintamai
Koontongkaew, Sittichai
Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title_full Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title_fullStr Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title_full_unstemmed Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title_short Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
title_sort sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (mc3t3-e1)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395237/
https://www.ncbi.nlm.nih.gov/pubmed/30895008
http://dx.doi.org/10.1016/j.jds.2016.07.004
work_keys_str_mv AT pramonosamdharu sandblastingandfibronectinderivedpeptideimmobilizationontitaniumsurfaceincreaseadhesionanddifferentiationofosteoblastlikecellsmc3t3e1
AT pugdeekamolparn sandblastingandfibronectinderivedpeptideimmobilizationontitaniumsurfaceincreaseadhesionanddifferentiationofosteoblastlikecellsmc3t3e1
AT suwanprateepjintamai sandblastingandfibronectinderivedpeptideimmobilizationontitaniumsurfaceincreaseadhesionanddifferentiationofosteoblastlikecellsmc3t3e1
AT koontongkaewsittichai sandblastingandfibronectinderivedpeptideimmobilizationontitaniumsurfaceincreaseadhesionanddifferentiationofosteoblastlikecellsmc3t3e1