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Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated os...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Association for Dental Sciences of the Republic of China
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395237/ https://www.ncbi.nlm.nih.gov/pubmed/30895008 http://dx.doi.org/10.1016/j.jds.2016.07.004 |
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author | Pramono, Samdharu Pugdee, Kamolparn Suwanprateep, Jintamai Koontongkaew, Sittichai |
author_facet | Pramono, Samdharu Pugdee, Kamolparn Suwanprateep, Jintamai Koontongkaew, Sittichai |
author_sort | Pramono, Samdharu |
collection | PubMed |
description | BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated osteoblast-like cell adhesion, spreading, proliferation, and differentiation. MATERIALS AND METHODS: Osteoblast-like cells (MC3T3-E1) were cultured on sandblasted Ti disks immobilized with FN or FN-derived peptides [GRGDSP (Gly-Arg-Gly-Asp-Ser), PHSRN (Pro-His-Ser-Arg-Asn), or GRGDSP/PHSRN]. Surface topography, cell morphology, cell adhesion, cell proliferation, analysis of osteogenesis-related genes and protein expression, alkaline phosphatase, and alizarin red staining of mineralization were evaluated. RESULTS: The sandblasted Ti coated with FN or FN-derived peptides enhanced cell adhesion and cell proliferation. However, the Ti coated with FN or FN-derived peptides groups were similar in cell spreading. Osteogenic differentiation was observed in the peptide-modified Ti surface groups, compared with that of the noncoated Ti group. FN and GRGDSP/PHSRN coating enhanced the gene and protein expression of Runx2, osteocalcin, and bone sialoprotein. Alkaline phosphatase activity and matrix mineralization were also markedly enhanced in the Ti coated groups. CONCLUSION: The sandblasted Ti coated with FN or FN-derived peptides (GRGDSP/PHSRN) markedly enhance adhesion, proliferation, and differentiation of osteoblast-like cells compared with uncoated sandblasted Ti. |
format | Online Article Text |
id | pubmed-6395237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Association for Dental Sciences of the Republic of China |
record_format | MEDLINE/PubMed |
spelling | pubmed-63952372019-03-20 Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) Pramono, Samdharu Pugdee, Kamolparn Suwanprateep, Jintamai Koontongkaew, Sittichai J Dent Sci Original Article BACKGROUND/PURPOSE: Various chemical titanium (Ti) surface modifications have been reported for enhancing cellular activities that promote early osseointegration. The purpose of this study was to determine if sandblasted Ti coated with or without fibronectin (FN) or FN-derived peptides stimulated osteoblast-like cell adhesion, spreading, proliferation, and differentiation. MATERIALS AND METHODS: Osteoblast-like cells (MC3T3-E1) were cultured on sandblasted Ti disks immobilized with FN or FN-derived peptides [GRGDSP (Gly-Arg-Gly-Asp-Ser), PHSRN (Pro-His-Ser-Arg-Asn), or GRGDSP/PHSRN]. Surface topography, cell morphology, cell adhesion, cell proliferation, analysis of osteogenesis-related genes and protein expression, alkaline phosphatase, and alizarin red staining of mineralization were evaluated. RESULTS: The sandblasted Ti coated with FN or FN-derived peptides enhanced cell adhesion and cell proliferation. However, the Ti coated with FN or FN-derived peptides groups were similar in cell spreading. Osteogenic differentiation was observed in the peptide-modified Ti surface groups, compared with that of the noncoated Ti group. FN and GRGDSP/PHSRN coating enhanced the gene and protein expression of Runx2, osteocalcin, and bone sialoprotein. Alkaline phosphatase activity and matrix mineralization were also markedly enhanced in the Ti coated groups. CONCLUSION: The sandblasted Ti coated with FN or FN-derived peptides (GRGDSP/PHSRN) markedly enhance adhesion, proliferation, and differentiation of osteoblast-like cells compared with uncoated sandblasted Ti. Association for Dental Sciences of the Republic of China 2016-12 2016-11-09 /pmc/articles/PMC6395237/ /pubmed/30895008 http://dx.doi.org/10.1016/j.jds.2016.07.004 Text en Copyright © 2016, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Pramono, Samdharu Pugdee, Kamolparn Suwanprateep, Jintamai Koontongkaew, Sittichai Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title | Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title_full | Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title_fullStr | Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title_full_unstemmed | Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title_short | Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) |
title_sort | sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (mc3t3-e1) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395237/ https://www.ncbi.nlm.nih.gov/pubmed/30895008 http://dx.doi.org/10.1016/j.jds.2016.07.004 |
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