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Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study

BACKGROUND/PURPOSE: The presence of adequate bone volume is a critical factor in rehabilitative dentistry. Despite the use of many promising alloplasts, success in stimulating bone formation has been limited, mostly due to poor local biological response. Growth factors have been introduced to stimul...

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Autores principales: Bedeloğlu, Elçin, Ersanlı, Selim, Arısan, Volkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association for Dental Sciences of the Republic of China 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395273/
https://www.ncbi.nlm.nih.gov/pubmed/30895017
http://dx.doi.org/10.1016/j.jds.2016.04.005
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author Bedeloğlu, Elçin
Ersanlı, Selim
Arısan, Volkan
author_facet Bedeloğlu, Elçin
Ersanlı, Selim
Arısan, Volkan
author_sort Bedeloğlu, Elçin
collection PubMed
description BACKGROUND/PURPOSE: The presence of adequate bone volume is a critical factor in rehabilitative dentistry. Despite the use of many promising alloplasts, success in stimulating bone formation has been limited, mostly due to poor local biological response. Growth factors have been introduced to stimulate angiogenesis and new bone formation. This histologic and histomorphometric study aimed to evaluate the effect of vascular endothelial growth factor (VEGF) and a biphasic alloplastic graft material (BA) on the healing of endosseous defects in rats. MATERIALS AND METHODS: Twenty male Wistar rats were used. Two critical-sized bone defects were created in both the right and left femurs of each rat. Each defect was randomly assigned to be treated with VEGF, BA, or VEGF + BA, or to be left empty as a control. Half of the animals were sacrificed after 1 week, and the remaining half were sacrificed after 2 weeks. Inflammation, necrosis, and new bone areas were evaluated by means of histologic and histomorphometric analyses. RESULTS: Compared to the control group, defects treated with VEGF alone or in combination with BA showed higher rates of bone formation (33.10–46.60%) on Day 7. Additionally, VEGF significantly reduced inflammation and necrosis (P < 0.001). However, the differences were no longer discernable on Day 14. CONCLUSION: VEGF makes a significant contribution to angiogenesis and osteogenesis in the early stages of bone defect healing, and its combination with an osteoconductive grafting material (BA) may further enhance new bone formation.
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spelling pubmed-63952732019-03-20 Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study Bedeloğlu, Elçin Ersanlı, Selim Arısan, Volkan J Dent Sci Original Article BACKGROUND/PURPOSE: The presence of adequate bone volume is a critical factor in rehabilitative dentistry. Despite the use of many promising alloplasts, success in stimulating bone formation has been limited, mostly due to poor local biological response. Growth factors have been introduced to stimulate angiogenesis and new bone formation. This histologic and histomorphometric study aimed to evaluate the effect of vascular endothelial growth factor (VEGF) and a biphasic alloplastic graft material (BA) on the healing of endosseous defects in rats. MATERIALS AND METHODS: Twenty male Wistar rats were used. Two critical-sized bone defects were created in both the right and left femurs of each rat. Each defect was randomly assigned to be treated with VEGF, BA, or VEGF + BA, or to be left empty as a control. Half of the animals were sacrificed after 1 week, and the remaining half were sacrificed after 2 weeks. Inflammation, necrosis, and new bone areas were evaluated by means of histologic and histomorphometric analyses. RESULTS: Compared to the control group, defects treated with VEGF alone or in combination with BA showed higher rates of bone formation (33.10–46.60%) on Day 7. Additionally, VEGF significantly reduced inflammation and necrosis (P < 0.001). However, the differences were no longer discernable on Day 14. CONCLUSION: VEGF makes a significant contribution to angiogenesis and osteogenesis in the early stages of bone defect healing, and its combination with an osteoconductive grafting material (BA) may further enhance new bone formation. Association for Dental Sciences of the Republic of China 2017-03 2016-07-09 /pmc/articles/PMC6395273/ /pubmed/30895017 http://dx.doi.org/10.1016/j.jds.2016.04.005 Text en © 2017 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Bedeloğlu, Elçin
Ersanlı, Selim
Arısan, Volkan
Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title_full Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title_fullStr Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title_full_unstemmed Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title_short Vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: An experimental rat study
title_sort vascular endothelial growth factor and biphasic calcium phosphate in the endosseous healing of femoral defects: an experimental rat study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395273/
https://www.ncbi.nlm.nih.gov/pubmed/30895017
http://dx.doi.org/10.1016/j.jds.2016.04.005
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