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EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis

The mechanical strength of inflorescence stems is an important trait in cut flowers. Calcium ions (Ca(2+)) play a pivotal role in maintaining stem strength, but little is known about the underlying molecular mechanisms. In this study, we treated herbaceous peony (Paeonia lactiflora Pall.) with ethyl...

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Autores principales: Tang, Yuhan, Zhao, Daqiu, Meng, Jiasong, Tao, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395589/
https://www.ncbi.nlm.nih.gov/pubmed/30854212
http://dx.doi.org/10.1038/s41438-019-0117-7
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author Tang, Yuhan
Zhao, Daqiu
Meng, Jiasong
Tao, Jun
author_facet Tang, Yuhan
Zhao, Daqiu
Meng, Jiasong
Tao, Jun
author_sort Tang, Yuhan
collection PubMed
description The mechanical strength of inflorescence stems is an important trait in cut flowers. Calcium ions (Ca(2+)) play a pivotal role in maintaining stem strength, but little is known about the underlying molecular mechanisms. In this study, we treated herbaceous peony (Paeonia lactiflora Pall.) with ethyl glycol tetraacetic acid (EGTA), an effective Ca(2+) chelator, and used morphology indicators, spectroscopic analysis, histochemical staining, electron microscopy, and proteomic techniques to investigate the role of Ca(2+) in inflorescence stem mechanical strength. The EGTA treatment reduced the mechanical strength of inflorescence stems, triggered the loss of Ca(2+) from cell walls, and reduced lignin in thickened secondary walls in xylem cells as determined by spectroscopic analysis and histochemical staining. Electron microscopy showed that the EGTA treatment also resulted in significantly fewer xylem cell layers with thickened secondary walls as well as in reducing the thickness of these secondary walls. The proteomic analysis showed 1065 differentially expressed proteins (DEPs) at the full-flowering stage (S4). By overlapping the Kyoto encyclopedia of genes and genomes (KEGG) and gene ontology (GO) analysis results, we identified 43 DEPs involved in signal transduction, transport, energy metabolism, carbohydrate metabolism, and secondary metabolite biosynthesis. Using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, we showed that EGTA treatment inhibited Ca(2+) sensors and secondary wall biosynthesis-related genes. Our findings revealed that EGTA treatment reduced the inflorescence stem mechanical strength by reducing lignin deposition in xylem cells through altering the expression of genes involved in Ca(2+) binding and secondary wall biosynthesis.
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spelling pubmed-63955892019-03-08 EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis Tang, Yuhan Zhao, Daqiu Meng, Jiasong Tao, Jun Hortic Res Article The mechanical strength of inflorescence stems is an important trait in cut flowers. Calcium ions (Ca(2+)) play a pivotal role in maintaining stem strength, but little is known about the underlying molecular mechanisms. In this study, we treated herbaceous peony (Paeonia lactiflora Pall.) with ethyl glycol tetraacetic acid (EGTA), an effective Ca(2+) chelator, and used morphology indicators, spectroscopic analysis, histochemical staining, electron microscopy, and proteomic techniques to investigate the role of Ca(2+) in inflorescence stem mechanical strength. The EGTA treatment reduced the mechanical strength of inflorescence stems, triggered the loss of Ca(2+) from cell walls, and reduced lignin in thickened secondary walls in xylem cells as determined by spectroscopic analysis and histochemical staining. Electron microscopy showed that the EGTA treatment also resulted in significantly fewer xylem cell layers with thickened secondary walls as well as in reducing the thickness of these secondary walls. The proteomic analysis showed 1065 differentially expressed proteins (DEPs) at the full-flowering stage (S4). By overlapping the Kyoto encyclopedia of genes and genomes (KEGG) and gene ontology (GO) analysis results, we identified 43 DEPs involved in signal transduction, transport, energy metabolism, carbohydrate metabolism, and secondary metabolite biosynthesis. Using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, we showed that EGTA treatment inhibited Ca(2+) sensors and secondary wall biosynthesis-related genes. Our findings revealed that EGTA treatment reduced the inflorescence stem mechanical strength by reducing lignin deposition in xylem cells through altering the expression of genes involved in Ca(2+) binding and secondary wall biosynthesis. Nature Publishing Group UK 2019-03-01 /pmc/articles/PMC6395589/ /pubmed/30854212 http://dx.doi.org/10.1038/s41438-019-0117-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tang, Yuhan
Zhao, Daqiu
Meng, Jiasong
Tao, Jun
EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title_full EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title_fullStr EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title_full_unstemmed EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title_short EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
title_sort egta reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395589/
https://www.ncbi.nlm.nih.gov/pubmed/30854212
http://dx.doi.org/10.1038/s41438-019-0117-7
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