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Mycobacterium ulcerans mycolactones-fungi crosstalking

The opportunistic pathogen Mycobacterium ulcerans, which is responsible for Buruli ulcer, synthesizes a series of plasmid-encoded macrolide exotoxins termed mycolactones. These toxins destabilize cell membranes and induce apoptosis-associated pleiotropic effects including tissue destruction, analges...

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Autores principales: Hammoudi, Nassim, Cassagne, Carole, Armstrong, Nicholas, Ranque, Stéphane, Henrissat, Bernard, Drancourt, Michel, Bouam, Amar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395692/
https://www.ncbi.nlm.nih.gov/pubmed/30816261
http://dx.doi.org/10.1038/s41598-019-39927-3
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author Hammoudi, Nassim
Cassagne, Carole
Armstrong, Nicholas
Ranque, Stéphane
Henrissat, Bernard
Drancourt, Michel
Bouam, Amar
author_facet Hammoudi, Nassim
Cassagne, Carole
Armstrong, Nicholas
Ranque, Stéphane
Henrissat, Bernard
Drancourt, Michel
Bouam, Amar
author_sort Hammoudi, Nassim
collection PubMed
description The opportunistic pathogen Mycobacterium ulcerans, which is responsible for Buruli ulcer, synthesizes a series of plasmid-encoded macrolide exotoxins termed mycolactones. These toxins destabilize cell membranes and induce apoptosis-associated pleiotropic effects including tissue destruction, analgesic and anti-inflammatory effects. Despite its medical interest, M. ulcerans is primarily an environmental mycobacterium and the primary functions of mycolactones in the natural ecosystems are unknown. High throughput biochemical profiling findings suggested that M. ulcerans may interact with fungi. Here, we report that semi-purified and purified mycolactones significantly enhance spore germination of Scedosporium apiospermum, Fusarium equiseti and Mucor circinelloides; and that M. ulcerans mycolactones significantly attract colonies of M. circinelloides whereas no significant effect was observed on S. apiospermum and F. equiseti. These experimental results suggest that mycolactones exhibit a chemoattractant activity independent of their cytotoxicity. In natural ecosystems, M. ulcerans mycolactones may act as spore germination inducers and chemoattractants for some fungi, suggesting a novel role for this unique class of mycobacterial toxins in natural ecosystems.
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spelling pubmed-63956922019-03-04 Mycobacterium ulcerans mycolactones-fungi crosstalking Hammoudi, Nassim Cassagne, Carole Armstrong, Nicholas Ranque, Stéphane Henrissat, Bernard Drancourt, Michel Bouam, Amar Sci Rep Article The opportunistic pathogen Mycobacterium ulcerans, which is responsible for Buruli ulcer, synthesizes a series of plasmid-encoded macrolide exotoxins termed mycolactones. These toxins destabilize cell membranes and induce apoptosis-associated pleiotropic effects including tissue destruction, analgesic and anti-inflammatory effects. Despite its medical interest, M. ulcerans is primarily an environmental mycobacterium and the primary functions of mycolactones in the natural ecosystems are unknown. High throughput biochemical profiling findings suggested that M. ulcerans may interact with fungi. Here, we report that semi-purified and purified mycolactones significantly enhance spore germination of Scedosporium apiospermum, Fusarium equiseti and Mucor circinelloides; and that M. ulcerans mycolactones significantly attract colonies of M. circinelloides whereas no significant effect was observed on S. apiospermum and F. equiseti. These experimental results suggest that mycolactones exhibit a chemoattractant activity independent of their cytotoxicity. In natural ecosystems, M. ulcerans mycolactones may act as spore germination inducers and chemoattractants for some fungi, suggesting a novel role for this unique class of mycobacterial toxins in natural ecosystems. Nature Publishing Group UK 2019-02-28 /pmc/articles/PMC6395692/ /pubmed/30816261 http://dx.doi.org/10.1038/s41598-019-39927-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hammoudi, Nassim
Cassagne, Carole
Armstrong, Nicholas
Ranque, Stéphane
Henrissat, Bernard
Drancourt, Michel
Bouam, Amar
Mycobacterium ulcerans mycolactones-fungi crosstalking
title Mycobacterium ulcerans mycolactones-fungi crosstalking
title_full Mycobacterium ulcerans mycolactones-fungi crosstalking
title_fullStr Mycobacterium ulcerans mycolactones-fungi crosstalking
title_full_unstemmed Mycobacterium ulcerans mycolactones-fungi crosstalking
title_short Mycobacterium ulcerans mycolactones-fungi crosstalking
title_sort mycobacterium ulcerans mycolactones-fungi crosstalking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395692/
https://www.ncbi.nlm.nih.gov/pubmed/30816261
http://dx.doi.org/10.1038/s41598-019-39927-3
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