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MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1
Several studies have shown that microRNA-375 (miR-375) is frequently downregulated in several types of human cancer including gastric cancer, colorectal cancer and oral squamous cell carcinoma. However, the role of miR-375 in human esophageal cancer remains unknown. In the current study, the express...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396021/ https://www.ncbi.nlm.nih.gov/pubmed/30867685 http://dx.doi.org/10.3892/etm.2018.7106 |
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author | Xu, Hui Jiang, Jialong Zhang, Jingjun Cheng, Liang Pan, Song Li, Yuanhai |
author_facet | Xu, Hui Jiang, Jialong Zhang, Jingjun Cheng, Liang Pan, Song Li, Yuanhai |
author_sort | Xu, Hui |
collection | PubMed |
description | Several studies have shown that microRNA-375 (miR-375) is frequently downregulated in several types of human cancer including gastric cancer, colorectal cancer and oral squamous cell carcinoma. However, the role of miR-375 in human esophageal cancer remains unknown. In the current study, the expression level of miR-375 was analyzed in 43 esophageal squamous cell carcinoma (ESCC) tissue and matched adjacent normal tissue samples from patients with ESCC by reverse transcription-quantitative polymerase chain reaction. In addition, the expression level of miR-375 was analyzed in ESCC cell lines (KYSE450 and KYSE150) and the human esophageal epithelial cell line Het-1A by the same method. The expression level of miR-375 was significantly downregulated in ESCC tissue samples and cell lines compared with adjacent normal tissue samples and the human esophageal epithelial cell line, respectively. The effect of miR-375 on ESCC cell proliferation was detected by cell counting kit-8 (CCK-8) and colony formation assays. miR-375 overexpression significantly decreased ESCC cell proliferation and colony formation. Bioinformatics analysis was used to predict specificity protein 1 (SP1) as a target gene of miR-375 in ESCC, and this was verified by dual-luciferase assay. The present study demonstrated that SP1 regulates ESCC cell proliferation and colony formation through direct interaction with miR-375. In addition, the overall survival of patients with ESCC was analyzed using the Kaplan-Meier method and log-rank test. The results indicated that patients with ESCC with high miR-375 expression had a better survival rate compared with patients with ESCC with low miR-375 expression. Taken together, these results suggest that downregulated miR-375 promotes ESCC cell proliferation and colony formation via direct targeting of SP1, and this association may contribute to ESCC progression. |
format | Online Article Text |
id | pubmed-6396021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63960212019-03-13 MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 Xu, Hui Jiang, Jialong Zhang, Jingjun Cheng, Liang Pan, Song Li, Yuanhai Exp Ther Med Articles Several studies have shown that microRNA-375 (miR-375) is frequently downregulated in several types of human cancer including gastric cancer, colorectal cancer and oral squamous cell carcinoma. However, the role of miR-375 in human esophageal cancer remains unknown. In the current study, the expression level of miR-375 was analyzed in 43 esophageal squamous cell carcinoma (ESCC) tissue and matched adjacent normal tissue samples from patients with ESCC by reverse transcription-quantitative polymerase chain reaction. In addition, the expression level of miR-375 was analyzed in ESCC cell lines (KYSE450 and KYSE150) and the human esophageal epithelial cell line Het-1A by the same method. The expression level of miR-375 was significantly downregulated in ESCC tissue samples and cell lines compared with adjacent normal tissue samples and the human esophageal epithelial cell line, respectively. The effect of miR-375 on ESCC cell proliferation was detected by cell counting kit-8 (CCK-8) and colony formation assays. miR-375 overexpression significantly decreased ESCC cell proliferation and colony formation. Bioinformatics analysis was used to predict specificity protein 1 (SP1) as a target gene of miR-375 in ESCC, and this was verified by dual-luciferase assay. The present study demonstrated that SP1 regulates ESCC cell proliferation and colony formation through direct interaction with miR-375. In addition, the overall survival of patients with ESCC was analyzed using the Kaplan-Meier method and log-rank test. The results indicated that patients with ESCC with high miR-375 expression had a better survival rate compared with patients with ESCC with low miR-375 expression. Taken together, these results suggest that downregulated miR-375 promotes ESCC cell proliferation and colony formation via direct targeting of SP1, and this association may contribute to ESCC progression. D.A. Spandidos 2019-03 2018-12-17 /pmc/articles/PMC6396021/ /pubmed/30867685 http://dx.doi.org/10.3892/etm.2018.7106 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Xu, Hui Jiang, Jialong Zhang, Jingjun Cheng, Liang Pan, Song Li, Yuanhai MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title | MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title_full | MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title_fullStr | MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title_full_unstemmed | MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title_short | MicroRNA-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1 |
title_sort | microrna-375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of sp1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396021/ https://www.ncbi.nlm.nih.gov/pubmed/30867685 http://dx.doi.org/10.3892/etm.2018.7106 |
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