Cargando…
Molecular genotyping of clinically important blood group antigens in patients with thalassaemia
BACKGROUND & OBJECTIVES: In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396563/ https://www.ncbi.nlm.nih.gov/pubmed/30778005 http://dx.doi.org/10.4103/ijmr.IJMR_455_17 |
_version_ | 1783399277776601088 |
---|---|
author | Kulkarni, Swati Choudhary, Bhavika Gogri, Harita Patil, Shashikant Manglani, Mamta Sharma, Ratna Madkaikar, Manisha |
author_facet | Kulkarni, Swati Choudhary, Bhavika Gogri, Harita Patil, Shashikant Manglani, Mamta Sharma, Ratna Madkaikar, Manisha |
author_sort | Kulkarni, Swati |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the correct antigen profile of these patients. This will facilitate the procurement of antigen-matched blood for transfusion to multitransfused patients. Thus, the aim of this study was to compare the serological phenotyping of common and clinically important antigens of Rh, Duffy, Kell, Kidd and MNS blood group systems with molecular genotyping amongst multitransfused thalassaemic patients. METHODS: Blood samples from 200 patients with thalassaemia and 100 ‘O’ group regular blood donors were tested using standard serological techniques and polymerase chain reaction-based methods for common antigens/alleles (C, c, D, E, e, Fy(a), Fy(b), Jk(a), Jk(b), K, k, M, N, S, s). RESULTS: Genotyping and phenotyping results were discordant in 77 per cent of thalassaemic patients for five pairs of antithetical antigens of Rh, Duffy, Kell and Kidd blood group systems. In the MNS blood group system, 59.1 per cent of patients showed discrepancy. The rate of alloimmunization among thalassaemics was 7.5 per cent. INTERPRETATION & CONCLUSIONS: Molecular genotyping enabled the determination of the actual antigen profile in multitransfused thalassaemia patients. This would help reduce the problem of alloimmunization in such patients and would also aid in the better management of transfusion therapy. |
format | Online Article Text |
id | pubmed-6396563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63965632019-03-25 Molecular genotyping of clinically important blood group antigens in patients with thalassaemia Kulkarni, Swati Choudhary, Bhavika Gogri, Harita Patil, Shashikant Manglani, Mamta Sharma, Ratna Madkaikar, Manisha Indian J Med Res Original Article BACKGROUND & OBJECTIVES: In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the correct antigen profile of these patients. This will facilitate the procurement of antigen-matched blood for transfusion to multitransfused patients. Thus, the aim of this study was to compare the serological phenotyping of common and clinically important antigens of Rh, Duffy, Kell, Kidd and MNS blood group systems with molecular genotyping amongst multitransfused thalassaemic patients. METHODS: Blood samples from 200 patients with thalassaemia and 100 ‘O’ group regular blood donors were tested using standard serological techniques and polymerase chain reaction-based methods for common antigens/alleles (C, c, D, E, e, Fy(a), Fy(b), Jk(a), Jk(b), K, k, M, N, S, s). RESULTS: Genotyping and phenotyping results were discordant in 77 per cent of thalassaemic patients for five pairs of antithetical antigens of Rh, Duffy, Kell and Kidd blood group systems. In the MNS blood group system, 59.1 per cent of patients showed discrepancy. The rate of alloimmunization among thalassaemics was 7.5 per cent. INTERPRETATION & CONCLUSIONS: Molecular genotyping enabled the determination of the actual antigen profile in multitransfused thalassaemia patients. This would help reduce the problem of alloimmunization in such patients and would also aid in the better management of transfusion therapy. Medknow Publications & Media Pvt Ltd 2018-12 /pmc/articles/PMC6396563/ /pubmed/30778005 http://dx.doi.org/10.4103/ijmr.IJMR_455_17 Text en Copyright: © 2019 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Kulkarni, Swati Choudhary, Bhavika Gogri, Harita Patil, Shashikant Manglani, Mamta Sharma, Ratna Madkaikar, Manisha Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title | Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title_full | Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title_fullStr | Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title_full_unstemmed | Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title_short | Molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
title_sort | molecular genotyping of clinically important blood group antigens in patients with thalassaemia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396563/ https://www.ncbi.nlm.nih.gov/pubmed/30778005 http://dx.doi.org/10.4103/ijmr.IJMR_455_17 |
work_keys_str_mv | AT kulkarniswati moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT choudharybhavika moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT gogriharita moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT patilshashikant moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT manglanimamta moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT sharmaratna moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia AT madkaikarmanisha moleculargenotypingofclinicallyimportantbloodgroupantigensinpatientswiththalassaemia |