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No limit in interspecific hybridization in schistosomes: observation from a case report

Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of...

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Autores principales: Depaquit, Jérôme, Akhoundi, Mohammad, Haouchine, Djamel, Mantelet, Stéphane, Izri, Arezki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396650/
https://www.ncbi.nlm.nih.gov/pubmed/30821247
http://dx.doi.org/10.1051/parasite/2019010
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author Depaquit, Jérôme
Akhoundi, Mohammad
Haouchine, Djamel
Mantelet, Stéphane
Izri, Arezki
author_facet Depaquit, Jérôme
Akhoundi, Mohammad
Haouchine, Djamel
Mantelet, Stéphane
Izri, Arezki
author_sort Depaquit, Jérôme
collection PubMed
description Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future.
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spelling pubmed-63966502019-03-26 No limit in interspecific hybridization in schistosomes: observation from a case report Depaquit, Jérôme Akhoundi, Mohammad Haouchine, Djamel Mantelet, Stéphane Izri, Arezki Parasite Research Article Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. EDP Sciences 2019-03-01 /pmc/articles/PMC6396650/ /pubmed/30821247 http://dx.doi.org/10.1051/parasite/2019010 Text en © J. Depaquit et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Depaquit, Jérôme
Akhoundi, Mohammad
Haouchine, Djamel
Mantelet, Stéphane
Izri, Arezki
No limit in interspecific hybridization in schistosomes: observation from a case report
title No limit in interspecific hybridization in schistosomes: observation from a case report
title_full No limit in interspecific hybridization in schistosomes: observation from a case report
title_fullStr No limit in interspecific hybridization in schistosomes: observation from a case report
title_full_unstemmed No limit in interspecific hybridization in schistosomes: observation from a case report
title_short No limit in interspecific hybridization in schistosomes: observation from a case report
title_sort no limit in interspecific hybridization in schistosomes: observation from a case report
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396650/
https://www.ncbi.nlm.nih.gov/pubmed/30821247
http://dx.doi.org/10.1051/parasite/2019010
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