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Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa

BACKGROUND: The threat of drug-resistant Pseudomonas aeruginosa requires great efforts to develop highly effective and safe bactericide. OBJECTIVE: This study aimed to investigate the antibacterial activity and mechanism of silver nanoparticles (AgNPs) against multidrug-resistant P. aeruginosa. METH...

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Autores principales: Liao, Shijing, Zhang, Yapeng, Pan, Xuanhe, Zhu, Feizhou, Jiang, Congyuan, Liu, Qianqian, Cheng, Zhongyi, Dai, Gan, Wu, Guojun, Wang, Linqian, Chen, Liyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396885/
https://www.ncbi.nlm.nih.gov/pubmed/30880959
http://dx.doi.org/10.2147/IJN.S191340
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author Liao, Shijing
Zhang, Yapeng
Pan, Xuanhe
Zhu, Feizhou
Jiang, Congyuan
Liu, Qianqian
Cheng, Zhongyi
Dai, Gan
Wu, Guojun
Wang, Linqian
Chen, Liyu
author_facet Liao, Shijing
Zhang, Yapeng
Pan, Xuanhe
Zhu, Feizhou
Jiang, Congyuan
Liu, Qianqian
Cheng, Zhongyi
Dai, Gan
Wu, Guojun
Wang, Linqian
Chen, Liyu
author_sort Liao, Shijing
collection PubMed
description BACKGROUND: The threat of drug-resistant Pseudomonas aeruginosa requires great efforts to develop highly effective and safe bactericide. OBJECTIVE: This study aimed to investigate the antibacterial activity and mechanism of silver nanoparticles (AgNPs) against multidrug-resistant P. aeruginosa. METHODS: The antimicrobial effect of AgNPs on clinical isolates of resistant P. aeruginosa was assessed by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). In multidrug-resistant P. aeruginosa, the alterations of morphology and structure were observed by the transmission electron microscopy (TEM); the differentially expressed proteins were analyzed by quantitative proteomics; the production of reactive oxygen species (ROS) was assayed by H(2)DCF-DA staining; the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) was chemically measured and the apoptosis-like effect was determined by flow cytometry. RESULTS: Antimicrobial tests revealed that AgNPs had highly bactericidal effect on the drug-resistant or multidrug-resistant P. aeruginosa with the MIC range of 1.406–5.625 µg/mL and the MBC range of 2.813–5.625 µg/mL. TEM showed that AgNPs could enter the multidrug-resistant bacteria and impair their morphology and structure. The proteomics quantified that, in the AgNP-treated bacteria, the levels of SOD, CAT, and POD, such as alkyl hydroperoxide reductase and organic hydroperoxide resistance protein, were obviously high, as well as the significant upregulation of low oxygen regulatory oxidases, including cbb3-type cytochrome c oxidase subunit P2, N2, and O2. Further results confirmed the excessive production of ROS. The antioxidants, reduced glutathione and ascorbic acid, partially antagonized the antibacterial action of AgNPs. The apoptosis-like rate of AgNP-treated bacteria was remarkably higher than that of the untreated bacteria (P<0.01). CONCLUSION: This study proved that AgNPs could play antimicrobial roles on the multidrug-resistant P. aeruginosa in a concentration- and time-dependent manner. The main mechanism involves the disequilibrium of oxidation and antioxidation processes and the failure to eliminate the excessive ROS.
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spelling pubmed-63968852019-03-15 Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa Liao, Shijing Zhang, Yapeng Pan, Xuanhe Zhu, Feizhou Jiang, Congyuan Liu, Qianqian Cheng, Zhongyi Dai, Gan Wu, Guojun Wang, Linqian Chen, Liyu Int J Nanomedicine Original Research BACKGROUND: The threat of drug-resistant Pseudomonas aeruginosa requires great efforts to develop highly effective and safe bactericide. OBJECTIVE: This study aimed to investigate the antibacterial activity and mechanism of silver nanoparticles (AgNPs) against multidrug-resistant P. aeruginosa. METHODS: The antimicrobial effect of AgNPs on clinical isolates of resistant P. aeruginosa was assessed by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). In multidrug-resistant P. aeruginosa, the alterations of morphology and structure were observed by the transmission electron microscopy (TEM); the differentially expressed proteins were analyzed by quantitative proteomics; the production of reactive oxygen species (ROS) was assayed by H(2)DCF-DA staining; the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) was chemically measured and the apoptosis-like effect was determined by flow cytometry. RESULTS: Antimicrobial tests revealed that AgNPs had highly bactericidal effect on the drug-resistant or multidrug-resistant P. aeruginosa with the MIC range of 1.406–5.625 µg/mL and the MBC range of 2.813–5.625 µg/mL. TEM showed that AgNPs could enter the multidrug-resistant bacteria and impair their morphology and structure. The proteomics quantified that, in the AgNP-treated bacteria, the levels of SOD, CAT, and POD, such as alkyl hydroperoxide reductase and organic hydroperoxide resistance protein, were obviously high, as well as the significant upregulation of low oxygen regulatory oxidases, including cbb3-type cytochrome c oxidase subunit P2, N2, and O2. Further results confirmed the excessive production of ROS. The antioxidants, reduced glutathione and ascorbic acid, partially antagonized the antibacterial action of AgNPs. The apoptosis-like rate of AgNP-treated bacteria was remarkably higher than that of the untreated bacteria (P<0.01). CONCLUSION: This study proved that AgNPs could play antimicrobial roles on the multidrug-resistant P. aeruginosa in a concentration- and time-dependent manner. The main mechanism involves the disequilibrium of oxidation and antioxidation processes and the failure to eliminate the excessive ROS. Dove Medical Press 2019-02-25 /pmc/articles/PMC6396885/ /pubmed/30880959 http://dx.doi.org/10.2147/IJN.S191340 Text en © 2019 Liao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed
spellingShingle Original Research
Liao, Shijing
Zhang, Yapeng
Pan, Xuanhe
Zhu, Feizhou
Jiang, Congyuan
Liu, Qianqian
Cheng, Zhongyi
Dai, Gan
Wu, Guojun
Wang, Linqian
Chen, Liyu
Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title_full Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title_fullStr Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title_full_unstemmed Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title_short Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa
title_sort antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant pseudomonas aeruginosa
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396885/
https://www.ncbi.nlm.nih.gov/pubmed/30880959
http://dx.doi.org/10.2147/IJN.S191340
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