Cargando…
Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis
Sargentodoxa cuneata is a tropical plant used in traditional Chinese medicine to treat intestinal inflammation. In this study, 3, 4-dihydroxyphenylethyl alcohol glycoside (DAG) was purified from the stem of S. cuneata using macroporous resins and its bioactivity was also investigated. The adsorption...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397144/ https://www.ncbi.nlm.nih.gov/pubmed/30824734 http://dx.doi.org/10.1038/s41598-019-38926-8 |
_version_ | 1783399366808043520 |
---|---|
author | Li, Dihua Zhuo, Yuzhen Zhang, Qi Zhang, Lanqiu Zhang, Shukun Lv, Yuanshan Li, Caixia Cui, Lihua Guan, Xin Yang, Lei Wang, Ximo |
author_facet | Li, Dihua Zhuo, Yuzhen Zhang, Qi Zhang, Lanqiu Zhang, Shukun Lv, Yuanshan Li, Caixia Cui, Lihua Guan, Xin Yang, Lei Wang, Ximo |
author_sort | Li, Dihua |
collection | PubMed |
description | Sargentodoxa cuneata is a tropical plant used in traditional Chinese medicine to treat intestinal inflammation. In this study, 3, 4-dihydroxyphenylethyl alcohol glycoside (DAG) was purified from the stem of S. cuneata using macroporous resins and its bioactivity was also investigated. The adsorption/desorption of DAG on macroporous resins was investigated systematically. HPD300 resin was selected as the most suitable medium for DAG purification. Further dynamic absorption/desorption experiments on the HPD300 column were conducted to obtain the optimal parameters. To obtain more than 95% DAG, a second stage procedure was developed to purify the DAG using SiliaSphere C18 with 8% v/v acetonitrile through elution at low pressure. Further investigation showed that DAG pretreatment significantly reversed the shortening of colon length, the increase in the disease activity index (DAI) scores and histological damage in the colon. Moreover, DAG greatly increased SOD and GPx activities, significantly decreased MPO and MDA activities and reduced the levels of pro-inflammatory cytokines in the colon. Free radical scavenging activities of DAG were assessed using DPPH, with an IC50 value of 17.03 ug/mL. Additionally, DAG suppressed ROS and proinflammatory cytokine production in LPS-stimulated RAW 264.7 macrophages by suppressing activation of the ERK1/2 and NF-κB pathways. The results were indicative of the antioxidant and anti-inflammatory properties of DAG. When viewed together, these findings indicated that DAG can be used to expand future pharmacological research and to potentially treat colitis. |
format | Online Article Text |
id | pubmed-6397144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63971442019-03-05 Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis Li, Dihua Zhuo, Yuzhen Zhang, Qi Zhang, Lanqiu Zhang, Shukun Lv, Yuanshan Li, Caixia Cui, Lihua Guan, Xin Yang, Lei Wang, Ximo Sci Rep Article Sargentodoxa cuneata is a tropical plant used in traditional Chinese medicine to treat intestinal inflammation. In this study, 3, 4-dihydroxyphenylethyl alcohol glycoside (DAG) was purified from the stem of S. cuneata using macroporous resins and its bioactivity was also investigated. The adsorption/desorption of DAG on macroporous resins was investigated systematically. HPD300 resin was selected as the most suitable medium for DAG purification. Further dynamic absorption/desorption experiments on the HPD300 column were conducted to obtain the optimal parameters. To obtain more than 95% DAG, a second stage procedure was developed to purify the DAG using SiliaSphere C18 with 8% v/v acetonitrile through elution at low pressure. Further investigation showed that DAG pretreatment significantly reversed the shortening of colon length, the increase in the disease activity index (DAI) scores and histological damage in the colon. Moreover, DAG greatly increased SOD and GPx activities, significantly decreased MPO and MDA activities and reduced the levels of pro-inflammatory cytokines in the colon. Free radical scavenging activities of DAG were assessed using DPPH, with an IC50 value of 17.03 ug/mL. Additionally, DAG suppressed ROS and proinflammatory cytokine production in LPS-stimulated RAW 264.7 macrophages by suppressing activation of the ERK1/2 and NF-κB pathways. The results were indicative of the antioxidant and anti-inflammatory properties of DAG. When viewed together, these findings indicated that DAG can be used to expand future pharmacological research and to potentially treat colitis. Nature Publishing Group UK 2019-03-01 /pmc/articles/PMC6397144/ /pubmed/30824734 http://dx.doi.org/10.1038/s41598-019-38926-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Dihua Zhuo, Yuzhen Zhang, Qi Zhang, Lanqiu Zhang, Shukun Lv, Yuanshan Li, Caixia Cui, Lihua Guan, Xin Yang, Lei Wang, Ximo Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title | Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title_full | Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title_fullStr | Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title_full_unstemmed | Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title_short | Purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis |
title_sort | purification of 3, 4-dihydroxyphenylethyl alcohol glycoside from sargentodoxa cuneata (oliv.) rehd. et wils. and its protective effects against dss-induced colitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397144/ https://www.ncbi.nlm.nih.gov/pubmed/30824734 http://dx.doi.org/10.1038/s41598-019-38926-8 |
work_keys_str_mv | AT lidihua purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT zhuoyuzhen purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT zhangqi purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT zhanglanqiu purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT zhangshukun purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT lvyuanshan purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT licaixia purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT cuilihua purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT guanxin purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT yanglei purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis AT wangximo purificationof34dihydroxyphenylethylalcoholglycosidefromsargentodoxacuneataolivrehdetwilsanditsprotectiveeffectsagainstdssinducedcolitis |