Cargando…

Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera

Among the factors that contribute to the reduction of honeybee populations are the pesticides. These chemical compounds reach the hive through forager bees, and once there, they can be ingested by the larvae. We evaluated the effects of repeated larval exposure to neonicotinoid insecticide, both in...

Descripción completa

Detalles Bibliográficos
Autores principales: Tadei, Rafaela, Domingues, Caio E. C., Malaquias, José Bruno, Camilo, Erasnilson Vieira, Malaspina, Osmar, Silva-Zacarin, Elaine C. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397237/
https://www.ncbi.nlm.nih.gov/pubmed/30824742
http://dx.doi.org/10.1038/s41598-019-39383-z
_version_ 1783399388824993792
author Tadei, Rafaela
Domingues, Caio E. C.
Malaquias, José Bruno
Camilo, Erasnilson Vieira
Malaspina, Osmar
Silva-Zacarin, Elaine C. M.
author_facet Tadei, Rafaela
Domingues, Caio E. C.
Malaquias, José Bruno
Camilo, Erasnilson Vieira
Malaspina, Osmar
Silva-Zacarin, Elaine C. M.
author_sort Tadei, Rafaela
collection PubMed
description Among the factors that contribute to the reduction of honeybee populations are the pesticides. These chemical compounds reach the hive through forager bees, and once there, they can be ingested by the larvae. We evaluated the effects of repeated larval exposure to neonicotinoid insecticide, both in isolation and in combination with strobilurin fungicide, at environmentally relevant doses. The total consumption of the contaminated diet was 23.63 ng fungicide/larvae (pyraclostrobin) and 0.2364 ng insecticide/larvae (clothianidin). The effects on post-embryonic development were evaluated over time. Additionally, we assessed the survival pattern of worker bees after emergence, and the pesticides’ effects on the behavior of newly emerged workers and young workers. Young bees that were exposed to the fungicide and those subjected to co-exposure to both pesticides during larval phase showed behavioral changes. The insecticide, both in isolation and in combination with fungicide reduced the bees’ longevity; this effect of larval exposure to pesticides was stronger in bees that were exposed only to the insecticide. Although the larvae did not have sensitivity to exposure to pesticides, they showed later effects after emergence, which may compromise the dynamics of the colony, contributing to the reduction of the populations of bees in agroecosystems.
format Online
Article
Text
id pubmed-6397237
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-63972372019-03-05 Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera Tadei, Rafaela Domingues, Caio E. C. Malaquias, José Bruno Camilo, Erasnilson Vieira Malaspina, Osmar Silva-Zacarin, Elaine C. M. Sci Rep Article Among the factors that contribute to the reduction of honeybee populations are the pesticides. These chemical compounds reach the hive through forager bees, and once there, they can be ingested by the larvae. We evaluated the effects of repeated larval exposure to neonicotinoid insecticide, both in isolation and in combination with strobilurin fungicide, at environmentally relevant doses. The total consumption of the contaminated diet was 23.63 ng fungicide/larvae (pyraclostrobin) and 0.2364 ng insecticide/larvae (clothianidin). The effects on post-embryonic development were evaluated over time. Additionally, we assessed the survival pattern of worker bees after emergence, and the pesticides’ effects on the behavior of newly emerged workers and young workers. Young bees that were exposed to the fungicide and those subjected to co-exposure to both pesticides during larval phase showed behavioral changes. The insecticide, both in isolation and in combination with fungicide reduced the bees’ longevity; this effect of larval exposure to pesticides was stronger in bees that were exposed only to the insecticide. Although the larvae did not have sensitivity to exposure to pesticides, they showed later effects after emergence, which may compromise the dynamics of the colony, contributing to the reduction of the populations of bees in agroecosystems. Nature Publishing Group UK 2019-03-01 /pmc/articles/PMC6397237/ /pubmed/30824742 http://dx.doi.org/10.1038/s41598-019-39383-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tadei, Rafaela
Domingues, Caio E. C.
Malaquias, José Bruno
Camilo, Erasnilson Vieira
Malaspina, Osmar
Silva-Zacarin, Elaine C. M.
Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title_full Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title_fullStr Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title_full_unstemmed Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title_short Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera
title_sort late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in africanized apis mellifera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397237/
https://www.ncbi.nlm.nih.gov/pubmed/30824742
http://dx.doi.org/10.1038/s41598-019-39383-z
work_keys_str_mv AT tadeirafaela lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera
AT dominguescaioec lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera
AT malaquiasjosebruno lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera
AT camiloerasnilsonvieira lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera
AT malaspinaosmar lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera
AT silvazacarinelainecm lateeffectoflarvalcoexposuretotheinsecticideclothianidinandfungicidepyraclostrobininafricanizedapismellifera