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Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant

Fertilizers and herbicides are two major components in the agriculture system for achieving crop productivity. Massive use of orthophosphate fertilizers and herbicides poses threats to phosphate reserves and aids the evolution of herbicide tolerant weed biotypes. Phosphite (Phi), a phosphate analog,...

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Autores principales: Ram, Babu, Fartyal, Dhirendra, Sheri, Vijay, Varakumar, Panditi, Borphukan, Bhabesh, James, Donald, Yadav, Renu, Bhatt, Arun, Agrawal, Pawan K., Achary, V. Mohan M., Reddy, Malireddy K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397861/
https://www.ncbi.nlm.nih.gov/pubmed/30858852
http://dx.doi.org/10.3389/fpls.2019.00037
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author Ram, Babu
Fartyal, Dhirendra
Sheri, Vijay
Varakumar, Panditi
Borphukan, Bhabesh
James, Donald
Yadav, Renu
Bhatt, Arun
Agrawal, Pawan K.
Achary, V. Mohan M.
Reddy, Malireddy K.
author_facet Ram, Babu
Fartyal, Dhirendra
Sheri, Vijay
Varakumar, Panditi
Borphukan, Bhabesh
James, Donald
Yadav, Renu
Bhatt, Arun
Agrawal, Pawan K.
Achary, V. Mohan M.
Reddy, Malireddy K.
author_sort Ram, Babu
collection PubMed
description Fertilizers and herbicides are two major components in the agriculture system for achieving crop productivity. Massive use of orthophosphate fertilizers and herbicides poses threats to phosphate reserves and aids the evolution of herbicide tolerant weed biotypes. Phosphite (Phi), a phosphate analog, has been proposed as more beneficial than traditionally used phosphate fertilizers and herbicides in the agriculture. We developed phoA overexpressing transgenic rice that minimizes the phosphate loss and contributes to weed management in the agriculture. The phoA rice lines showed improved root, shoot length and total biomass production under phosphite conditions. Additionally, the complete phenotype and productivity of phoA lines under the phosphite treatment attained was similar to that of plants under phosphate sufficient condition. The Phi metabolizing properties of the phoA overexpressed lines improved under the Phi application and phi treatment enabled controlling of weeds without compromising the yield of transgenic rice plants. Our results indicated that phoA alone or in combination with other Phi metabolizing gene(s) can possibly be used as an effective ameliorating system for improving crop plants for phi-based fertilization and weed management strategy in the agriculture.
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spelling pubmed-63978612019-03-11 Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant Ram, Babu Fartyal, Dhirendra Sheri, Vijay Varakumar, Panditi Borphukan, Bhabesh James, Donald Yadav, Renu Bhatt, Arun Agrawal, Pawan K. Achary, V. Mohan M. Reddy, Malireddy K. Front Plant Sci Plant Science Fertilizers and herbicides are two major components in the agriculture system for achieving crop productivity. Massive use of orthophosphate fertilizers and herbicides poses threats to phosphate reserves and aids the evolution of herbicide tolerant weed biotypes. Phosphite (Phi), a phosphate analog, has been proposed as more beneficial than traditionally used phosphate fertilizers and herbicides in the agriculture. We developed phoA overexpressing transgenic rice that minimizes the phosphate loss and contributes to weed management in the agriculture. The phoA rice lines showed improved root, shoot length and total biomass production under phosphite conditions. Additionally, the complete phenotype and productivity of phoA lines under the phosphite treatment attained was similar to that of plants under phosphate sufficient condition. The Phi metabolizing properties of the phoA overexpressed lines improved under the Phi application and phi treatment enabled controlling of weeds without compromising the yield of transgenic rice plants. Our results indicated that phoA alone or in combination with other Phi metabolizing gene(s) can possibly be used as an effective ameliorating system for improving crop plants for phi-based fertilization and weed management strategy in the agriculture. Frontiers Media S.A. 2019-02-25 /pmc/articles/PMC6397861/ /pubmed/30858852 http://dx.doi.org/10.3389/fpls.2019.00037 Text en Copyright © 2019 Ram, Fartyal, Sheri, Varakumar, Borphukan, James, Yadav, Bhatt, Agrawal, Achary and Reddy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ram, Babu
Fartyal, Dhirendra
Sheri, Vijay
Varakumar, Panditi
Borphukan, Bhabesh
James, Donald
Yadav, Renu
Bhatt, Arun
Agrawal, Pawan K.
Achary, V. Mohan M.
Reddy, Malireddy K.
Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title_full Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title_fullStr Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title_full_unstemmed Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title_short Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant
title_sort characterization of phoa, a bacterial alkaline phosphatase for phi use efficiency in rice plant
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397861/
https://www.ncbi.nlm.nih.gov/pubmed/30858852
http://dx.doi.org/10.3389/fpls.2019.00037
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