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Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs
Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the pla...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397873/ https://www.ncbi.nlm.nih.gov/pubmed/30858855 http://dx.doi.org/10.3389/fpls.2019.00162 |
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author | Mücke, Stefanie Reschke, Maik Erkes, Annett Schwietzer, Claudia-Alice Becker, Sebastian Streubel, Jana Morgan, Richard D. Wilson, Geoffrey G. Grau, Jan Boch, Jens |
author_facet | Mücke, Stefanie Reschke, Maik Erkes, Annett Schwietzer, Claudia-Alice Becker, Sebastian Streubel, Jana Morgan, Richard D. Wilson, Geoffrey G. Grau, Jan Boch, Jens |
author_sort | Mücke, Stefanie |
collection | PubMed |
description | Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125(T) and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease. |
format | Online Article Text |
id | pubmed-6397873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63978732019-03-11 Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs Mücke, Stefanie Reschke, Maik Erkes, Annett Schwietzer, Claudia-Alice Becker, Sebastian Streubel, Jana Morgan, Richard D. Wilson, Geoffrey G. Grau, Jan Boch, Jens Front Plant Sci Plant Science Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125(T) and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease. Frontiers Media S.A. 2019-02-25 /pmc/articles/PMC6397873/ /pubmed/30858855 http://dx.doi.org/10.3389/fpls.2019.00162 Text en Copyright © 2019 Mücke, Reschke, Erkes, Schwietzer, Becker, Streubel, Morgan, Wilson, Grau and Boch. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mücke, Stefanie Reschke, Maik Erkes, Annett Schwietzer, Claudia-Alice Becker, Sebastian Streubel, Jana Morgan, Richard D. Wilson, Geoffrey G. Grau, Jan Boch, Jens Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title | Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title_full | Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title_fullStr | Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title_full_unstemmed | Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title_short | Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs |
title_sort | transcriptional reprogramming of rice cells by xanthomonas oryzae tales |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397873/ https://www.ncbi.nlm.nih.gov/pubmed/30858855 http://dx.doi.org/10.3389/fpls.2019.00162 |
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