Cargando…
Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer
Calponin 3 (CNN3) is an F-actin-binding protein that regulates actin cytoskeletal rearrangement. However, the role of CNN3 in cancer cell invasion and resistance to chemotherapeutic agents has not yet been investigated. The present study was undertaken to investigate whether CNN3 influences cancer-r...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398029/ https://www.ncbi.nlm.nih.gov/pubmed/30911294 http://dx.doi.org/10.1155/2019/3024970 |
_version_ | 1783399511849172992 |
---|---|
author | Hong, Keun-Seok Kim, Hyemin Kim, Seon-Hee Kim, Minju Yoo, Jiyun |
author_facet | Hong, Keun-Seok Kim, Hyemin Kim, Seon-Hee Kim, Minju Yoo, Jiyun |
author_sort | Hong, Keun-Seok |
collection | PubMed |
description | Calponin 3 (CNN3) is an F-actin-binding protein that regulates actin cytoskeletal rearrangement. However, the role of CNN3 in cancer cell invasion and resistance to chemotherapeutic agents has not yet been investigated. The present study was undertaken to investigate whether CNN3 influences cancer-related phenotypes in gastric cancer. We demonstrate that CNN3 contributes to cell invasion and resistance to doxorubicin in gastric cancer. CNN3 expression was markedly elevated in highly invasive cancer cell lines compared to less invasive or noninvasive cancer cell lines. Depletion of CNN3 protein suppressed the invasive ability of gastric cancer cells. The highly invasive MKN-28 gastric cancer cells were more resistant to doxorubicin than the noninvasive MKN-45 cells; however, knockdown of CNN3 expression in MKN-28 cells resensitized them to doxorubicin treatment. Taken together, our results suggest that CNN3 plays a key role in invasiveness and doxorubicin resistance in gastric cancer cells. |
format | Online Article Text |
id | pubmed-6398029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63980292019-03-25 Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer Hong, Keun-Seok Kim, Hyemin Kim, Seon-Hee Kim, Minju Yoo, Jiyun Gastroenterol Res Pract Research Article Calponin 3 (CNN3) is an F-actin-binding protein that regulates actin cytoskeletal rearrangement. However, the role of CNN3 in cancer cell invasion and resistance to chemotherapeutic agents has not yet been investigated. The present study was undertaken to investigate whether CNN3 influences cancer-related phenotypes in gastric cancer. We demonstrate that CNN3 contributes to cell invasion and resistance to doxorubicin in gastric cancer. CNN3 expression was markedly elevated in highly invasive cancer cell lines compared to less invasive or noninvasive cancer cell lines. Depletion of CNN3 protein suppressed the invasive ability of gastric cancer cells. The highly invasive MKN-28 gastric cancer cells were more resistant to doxorubicin than the noninvasive MKN-45 cells; however, knockdown of CNN3 expression in MKN-28 cells resensitized them to doxorubicin treatment. Taken together, our results suggest that CNN3 plays a key role in invasiveness and doxorubicin resistance in gastric cancer cells. Hindawi 2019-02-18 /pmc/articles/PMC6398029/ /pubmed/30911294 http://dx.doi.org/10.1155/2019/3024970 Text en Copyright © 2019 Keun-Seok Hong et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hong, Keun-Seok Kim, Hyemin Kim, Seon-Hee Kim, Minju Yoo, Jiyun Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title | Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title_full | Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title_fullStr | Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title_full_unstemmed | Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title_short | Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer |
title_sort | calponin 3 regulates cell invasion and doxorubicin resistance in gastric cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398029/ https://www.ncbi.nlm.nih.gov/pubmed/30911294 http://dx.doi.org/10.1155/2019/3024970 |
work_keys_str_mv | AT hongkeunseok calponin3regulatescellinvasionanddoxorubicinresistanceingastriccancer AT kimhyemin calponin3regulatescellinvasionanddoxorubicinresistanceingastriccancer AT kimseonhee calponin3regulatescellinvasionanddoxorubicinresistanceingastriccancer AT kimminju calponin3regulatescellinvasionanddoxorubicinresistanceingastriccancer AT yoojiyun calponin3regulatescellinvasionanddoxorubicinresistanceingastriccancer |