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Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer
BACKGROUND: Circulating miRNAs (miRs) are increasingly recognized as potential biomarkers in cancer. We aimed to evaluate the differential expression of miR-23b and miR-190 which are involved in tumor dormancy, miR-21 involved in metastasis and miR-200b and miR-200c involved in epithelial-mesenchyma...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398176/ https://www.ncbi.nlm.nih.gov/pubmed/30847025 http://dx.doi.org/10.18632/oncotarget.26629 |
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author | Papadaki, Chara Stoupis, Giannis Tsalikis, Leuteris Monastirioti, Alexia Papadaki, Maria Maliotis, Neofytos Stratigos, Michalis Mastrostamatis, Georgios Mavroudis, Dimitrios Agelaki, Sofia |
author_facet | Papadaki, Chara Stoupis, Giannis Tsalikis, Leuteris Monastirioti, Alexia Papadaki, Maria Maliotis, Neofytos Stratigos, Michalis Mastrostamatis, Georgios Mavroudis, Dimitrios Agelaki, Sofia |
author_sort | Papadaki, Chara |
collection | PubMed |
description | BACKGROUND: Circulating miRNAs (miRs) are increasingly recognized as potential biomarkers in cancer. We aimed to evaluate the differential expression of miR-23b and miR-190 which are involved in tumor dormancy, miR-21 involved in metastasis and miR-200b and miR-200c involved in epithelial-mesenchymal transition (EMT) and metastasis, in the plasma of patients with early and metastatic breast cancer (MBC). We also aimed to identify associations of the expression levels with patient and disease characteristics and outcomes in metastatic patients treated with first-line chemotherapy. RESULTS: miR-21 (p < 0.001), miR-23b (p = 0.033), miR-200b (p < 0.001) and miR-200c (p < 0.001) expression was higher in metastatic compared to early breast cancer. ROC curve analysis showed that miR-21 (AUC = 0.722; p < 0.001) and miR-200b (AUC = 0.720; p < 0.001) distinguished with high accuracy among the two disease states, whereas the combination of miR-21, miR-190, miR-200b and miR-200c, further improved accuracy (AUC = 0.797; p < 0.001). High miR-200b expression independently predicted for shorter OS (p = 0.026) in MBC. High expression of both miR23b and miR-190 emerged as a strong independent factor associated with shorter PFS (p = 0.001) in de novo metastatic patients and high miR-200b independently predicted for decreased OS in the HER2-negative subgroup (p = 0.007). MATERIALS AND METHODS: Blood samples were obtained from patients with early (n = 133) and MBC (n = 110) before adjuvant or first-line chemotherapy, respectively. Plasma miRNA expression levels were assessed by RT-qPCR and were classified as high or low according to the median values. CONCLUSIONS: Our results are in support of the concept that circulating miRNAs represent a tool with significant diagnostic and prognostic implications in breast cancer. |
format | Online Article Text |
id | pubmed-6398176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-63981762019-03-07 Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer Papadaki, Chara Stoupis, Giannis Tsalikis, Leuteris Monastirioti, Alexia Papadaki, Maria Maliotis, Neofytos Stratigos, Michalis Mastrostamatis, Georgios Mavroudis, Dimitrios Agelaki, Sofia Oncotarget Research Paper BACKGROUND: Circulating miRNAs (miRs) are increasingly recognized as potential biomarkers in cancer. We aimed to evaluate the differential expression of miR-23b and miR-190 which are involved in tumor dormancy, miR-21 involved in metastasis and miR-200b and miR-200c involved in epithelial-mesenchymal transition (EMT) and metastasis, in the plasma of patients with early and metastatic breast cancer (MBC). We also aimed to identify associations of the expression levels with patient and disease characteristics and outcomes in metastatic patients treated with first-line chemotherapy. RESULTS: miR-21 (p < 0.001), miR-23b (p = 0.033), miR-200b (p < 0.001) and miR-200c (p < 0.001) expression was higher in metastatic compared to early breast cancer. ROC curve analysis showed that miR-21 (AUC = 0.722; p < 0.001) and miR-200b (AUC = 0.720; p < 0.001) distinguished with high accuracy among the two disease states, whereas the combination of miR-21, miR-190, miR-200b and miR-200c, further improved accuracy (AUC = 0.797; p < 0.001). High miR-200b expression independently predicted for shorter OS (p = 0.026) in MBC. High expression of both miR23b and miR-190 emerged as a strong independent factor associated with shorter PFS (p = 0.001) in de novo metastatic patients and high miR-200b independently predicted for decreased OS in the HER2-negative subgroup (p = 0.007). MATERIALS AND METHODS: Blood samples were obtained from patients with early (n = 133) and MBC (n = 110) before adjuvant or first-line chemotherapy, respectively. Plasma miRNA expression levels were assessed by RT-qPCR and were classified as high or low according to the median values. CONCLUSIONS: Our results are in support of the concept that circulating miRNAs represent a tool with significant diagnostic and prognostic implications in breast cancer. Impact Journals LLC 2019-01-29 /pmc/articles/PMC6398176/ /pubmed/30847025 http://dx.doi.org/10.18632/oncotarget.26629 Text en Copyright: © 2019 Papadaki et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Papadaki, Chara Stoupis, Giannis Tsalikis, Leuteris Monastirioti, Alexia Papadaki, Maria Maliotis, Neofytos Stratigos, Michalis Mastrostamatis, Georgios Mavroudis, Dimitrios Agelaki, Sofia Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title | Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title_full | Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title_fullStr | Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title_full_unstemmed | Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title_short | Circulating miRNAs as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
title_sort | circulating mirnas as a marker of metastatic disease and prognostic factor in metastatic breast cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398176/ https://www.ncbi.nlm.nih.gov/pubmed/30847025 http://dx.doi.org/10.18632/oncotarget.26629 |
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