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Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity

The absence of new antibiotics is guiding more and more researchers to specific ecosystems. One hundred and sixty-three Actinobacteria isolates were isolated from Merzouga sand and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorg...

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Autores principales: Ouchari, Lahcen, Boukeskasse, Amal, Bouizgarne, Brahim, Ouhdouch, Yedir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398458/
https://www.ncbi.nlm.nih.gov/pubmed/30127092
http://dx.doi.org/10.1242/bio.035410
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author Ouchari, Lahcen
Boukeskasse, Amal
Bouizgarne, Brahim
Ouhdouch, Yedir
author_facet Ouchari, Lahcen
Boukeskasse, Amal
Bouizgarne, Brahim
Ouhdouch, Yedir
author_sort Ouchari, Lahcen
collection PubMed
description The absence of new antibiotics is guiding more and more researchers to specific ecosystems. One hundred and sixty-three Actinobacteria isolates were isolated from Merzouga sand and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as human potential pathogens were used. The electrophoretic profiles of isolates obtained by repetitive element PCR fingerprinting (rep-PCR) were compared by clustering. Results showed that among the tested isolates, 59% were active against one or more in testing Gram-positive, Gram-negative and the yeast Candida albicans. The importance of culture media for the activity expression was revealed. Comparative analysis of antimicrobial activity divided isolates into 15 groups. The comparison of the average diameters of inhibition zones using Minitab V.17 allowed subdivision of the 15 groups into 20 subgroups. Dendrograms derived from the BOXA1R-PCR fingerprints showed that 36 isolates were grouped in 16 clusters, containing from two to four isolates while 127 isolates were not grouped. The tested antimicrobial activities showed a high biological diversity with important inhibition of pathogens tested. The rep-PCR revealed a high taxonomic diversity of isolates. The combination of antimicrobial activity and rep-PCR results revealed the diverse pattern of Merzouga sand dune Actinobacteria.
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spelling pubmed-63984582019-03-05 Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity Ouchari, Lahcen Boukeskasse, Amal Bouizgarne, Brahim Ouhdouch, Yedir Biol Open Research Article The absence of new antibiotics is guiding more and more researchers to specific ecosystems. One hundred and sixty-three Actinobacteria isolates were isolated from Merzouga sand and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as human potential pathogens were used. The electrophoretic profiles of isolates obtained by repetitive element PCR fingerprinting (rep-PCR) were compared by clustering. Results showed that among the tested isolates, 59% were active against one or more in testing Gram-positive, Gram-negative and the yeast Candida albicans. The importance of culture media for the activity expression was revealed. Comparative analysis of antimicrobial activity divided isolates into 15 groups. The comparison of the average diameters of inhibition zones using Minitab V.17 allowed subdivision of the 15 groups into 20 subgroups. Dendrograms derived from the BOXA1R-PCR fingerprints showed that 36 isolates were grouped in 16 clusters, containing from two to four isolates while 127 isolates were not grouped. The tested antimicrobial activities showed a high biological diversity with important inhibition of pathogens tested. The rep-PCR revealed a high taxonomic diversity of isolates. The combination of antimicrobial activity and rep-PCR results revealed the diverse pattern of Merzouga sand dune Actinobacteria. The Company of Biologists Ltd 2018-08-20 /pmc/articles/PMC6398458/ /pubmed/30127092 http://dx.doi.org/10.1242/bio.035410 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Ouchari, Lahcen
Boukeskasse, Amal
Bouizgarne, Brahim
Ouhdouch, Yedir
Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title_full Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title_fullStr Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title_full_unstemmed Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title_short Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity
title_sort antimicrobial potential of actinomycetes isolated from the unexplored hot merzouga desert and their taxonomic diversity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398458/
https://www.ncbi.nlm.nih.gov/pubmed/30127092
http://dx.doi.org/10.1242/bio.035410
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